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506151 IRSp53, His•Tag® Fusion

IRSp53, His•Tag® Fusion MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Insulin Receptor Substrate p53, Human, Recombinant, IRSp53, Human, Recombinant

506151
  
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      Overview

      Replacement Information

      Key Spec Table

      Purity
      ≥90% by SDS-PAGE
      Description
      Overview

      This product has been discontinued.





      Full-length, recombinant, human insulin receptor substrate p53 (IRSp53) fused to His•Tag® and S•Tag™ sequences at the N-terminus and expressed in E. coli. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. IRSp53 (a.k.a. IRS-58, IRSp53/58, BAIP2, BAIAP2, FLAF3) is a secretin receptor family protein induced by p53. Yeh, et al. cloned a hamster cDNA encoding a 53 kD BAI-associated protein that was tyrosine phosphorylated by the insulin receptor when expressed in mammalian cells. They termed the protein IRSp53 for "insulin receptor substrate p53". The protein contains 521 amino acids with a calculated molecular mass of 57 kDa and is highly expressed in the brain. IRSp53 is an adapter protein that links membrane-bound small G-proteins to cytoplasmic effector proteins and is necessary for CDC42-mediated reorganization of the actin cytoskeleton and for RAC1-mediated membrane ruffling. The protein appears to be localized primarily in the particulate fraction of cell lysates. It is phosphorylated by insulin receptor (InsR) in response to insulin treatment. Phosphorylated sites include Tyr337/Tyr505 by InsR and Tyr471 by Src and EGFR. In addition, the SH3 domain (aa 374-437) interacts with ATN1, BAI1, WASF1, WASF2, SHANK1, DIAPH1, and ENAH.
      Catalogue Number506151
      Brand Family Calbiochem®
      SynonymsInsulin Receptor Substrate p53, Human, Recombinant, IRSp53, Human, Recombinant
      Application Data
      References
      ReferencesLennon, G., et al. 1996. Genomics. 33, 151.
      Yeh, T.C., et al. 1996. J. Biol. Chem. 271, 2921.
      Product Information
      FormLiquid
      FormulationIn PBS, 6 M Urea.
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Irritant
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      506151 0

      Documentation

      References

      Reference overview
      Lennon, G., et al. 1996. Genomics. 33, 151.
      Yeh, T.C., et al. 1996. J. Biol. Chem. 271, 2921.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-August-2008 RFH
      SynonymsInsulin Receptor Substrate p53, Human, Recombinant, IRSp53, Human, Recombinant
      Application Data
      DescriptionFull-length, recombinant, human insulin receptor substrate p53 (IRSp53) fused to His•Tag® and S•Tag™ sequences at the N-terminus and expressed in E. coli. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. IRSp53 (a.k.a. IRS-58, IRSp53/58, BAIP2, BAIAP2, FLAF3) is a secretin receptor family protein induced by p53. Yeh, et al. cloned a hamster cDNA encoding a 53 kD BAI-associated protein that was tyrosine phosphorylated by the insulin receptor when expressed in mammalian cells. They termed the protein IRSp53 for "insulin receptor substrate p53". The protein contains 521 amino acids with a calculated molecular mass of 57 kDa and is highly expressed in the brain. IRSp53 is an adapter protein that links membrane-bound small G-proteins to cytoplasmic effector proteins and is necessary for CDC42-mediated reorganization of the actin cytoskeleton and for RAC1-mediated membrane ruffling. The protein appears to be localized primarily in the particulate fraction of cell lysates. It is phosphorylated by insulin receptor (InsR) in response to insulin treatment. Phosphorylated sites include Tyr337/Tyr505 by InsR and Tyr471 by Src and EGFR. In addition, the SH3 domain (aa 374-437) interacts with ATN1, BAI1, WASF1, WASF2, SHANK1, DIAPH1, and ENAH.
      FormLiquid
      FormulationIn PBS, 6 M Urea.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥90% by SDS-PAGE
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Irritant
      ReferencesLennon, G., et al. 1996. Genomics. 33, 151.
      Yeh, T.C., et al. 1996. J. Biol. Chem. 271, 2921.