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CC095-5MG Sigma-AldrichLaminin, mouse purified

Laminins are the major noncollagenous constituent of basement membranes. This purified mouse laminin was isolated from Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a mouse tumor that produces large amounts of basement membranes.

Laminin, mouse purified MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
Data Sheet

Key Applications: Cell Culture UniProt Number: P07942 Entrez Gene Number: NM_002291.1

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CC095-5MG

5 mg

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Key Spec Table

Key Applications	Species	Source
CULT	Mouse	Isolated from Engelbreth-Holm-Swarm (EHS) mouse sarcoma

Description
Catalogue Number	CC095-5MG
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Laminin, mouse purified
Overview	Specificity:Molecular weight = 850 kD; 3 chains expressed as two major bands at 200 and 400 kD on SDS-PAGE. Preparation: Prepared from Engelbreth-Holm-Swarm {EHS} sarcoma according to the method of Templ et al 1979 {http://www.jbc.org/cgi/reprint/254/19/9933.pdf} Briefly, tumor homogenates were subjected to NaCl extractions and centrifugations. Laminin was found in the 0.5M NaCl fraction. After subsequent salt gradients and further centrifugation steps, the laminin was dialyzed against 2M urea and passed over a column of DEAE, followed by molecular sieve chromatography and concentration by ultrafiltration.

References

Product Information
Presentation	Purified protein. Liquid; in 0.05 M Tris-HCl, pH 7.4, containing 0.15 M NaCl. No preservatives.
Quality Level	MQ100

Applications
Application	Laminins are the major noncollagenous constituent of basement membranes. This purified mouse laminin was isolated from Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a mouse tumor that produces large amounts of basement membranes.
Key Applications	Cell Culture
Application Notes	Promotes the adhesion and growth of human carcinoma and sarcoma cells, human retinoblastoma cells, liver cells, mouse neuroblastoma cells, mouse embryonal carcinoma cells, and seems to be involved in the development of embryonal tissue. The optimal laminin concentration may vary with the cell type and has to be determined experimentally.

Biological Information
Concentration	1 mg/mL
Source	Isolated from Engelbreth-Holm-Swarm (EHS) mouse sarcoma
Entrez Gene Number	NM_002291.1
Entrez Gene Summary	Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis.
Gene Symbol	LAMB1 MGC142015 CLM
Purification Method	Purified according to the method of Timpl, et. al.
UniProt Number	P07942
UniProt Summary	FUNCTION: SwissProt: P07942 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. SIZE: 1786 amino acids; 198066 Da SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Beta-1 is a subunit of laminin-1 (EHS laminin), laminin-2 (merosin), and laminin-6 (K-laminin). SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component. DOMAIN: SwissProt: P07942 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI and IV are globular. SIMILARITY: Contains 13 laminin EGF-like domains. & Contains 1 laminin IV type B domain. & Contains 1 laminin N-terminal domain.
Stem Cell Type	Human Embryonic Stem Cells Mouse Embryonic Stem Cells Mesenchymal Stem Cells Neural Stem Cells Hematopoietic Stem Cells Epithelial Cells Pancreatic Stem Cells Induced Pluripotent Stem Cells

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store at -20°C for up to six months. Thaw product carefully at 2-8°C to avoid premature gelling, and do not refreeze. Once thawed. material should be stored at 2-8°C for up to 3 months.

Packaging Information
Material Size	5 mg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
CC095-5MG	04053252745027

Documentation

Laminin, mouse purified SDS

Title
Safety Data Sheet (SDS)

Laminin, mouse purified Certificates of Analysis

Title	Lot Number
MOUSE LAMININ PURIFIED PROTEIN - 2080294	2080294
MOUSE LAMININ PURIFIED PROTEIN - 2119000	2119000
MOUSE LAMININ PURIFIED PROTEIN - 2360598	2360598
MOUSE LAMININ PURIFIED PROTEIN - 2447738	2447738
MOUSE LAMININ - 3054628	3054628
MOUSE LAMININ - 3178592	3178592
MOUSE LAMININ - 3193850	3193850
MOUSE LAMININ - 3195937	3195937
MOUSE LAMININ - 3224650	3224650
MOUSE LAMININ - 3237490	3237490

Data Sheet

Title
MOUSE LAMININ PURIFIED PROTEIN

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CC095

Laminin, mouse purified

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