Millipore Sigma Vibrant Logo

HADK2MAG-61K MILLIPLEX MAP Human Adipokine Magnetic Bead Panel 2 - Endocrine Multiplex Assay

HADK2MAG-61K
Design And Price Your Kit

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
IL-1β, IL-6, IL-8, Insulin, Leptin, MCP-1, NGF, TNF-αHMplexLuminex xMAP
Description
Catalogue NumberHADK2MAG-61K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Adipokine Magnetic Bead Panel 2 - Endocrine Multiplex Assay
OverviewThe MILLIPLEX® MAP Human Adipokine Magnetic Bead Panel 2 is a 8-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples: IL-1β, IL-6, IL-8, Insulin, Leptin, MCP-1, NGF and TNF-α. This kit uses a 96-well format, contains a lyophilized standard cocktail, 2 quality controls and can measure up to 38 serum or plasma samples in duplicate.

References:
Cephalalgia. 2016 May 18. pii: 0333102416650705.
Evidence for potential involvement of pro-inflammatory adipokines in the pathogenesis of idiopathic intracranial hypertension.
Samancı B, Samancı Y, Tüzün E, Altıokka-Uzun G, Ekizoğlu E, İçöz S, Şahin E, Küçükali Cİ, Baykan B.
Background InformationPreviously the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete a number of hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • IL-1β: Intra-assay <10% CV Inter-assay <15% CV
  • IL-6: Intra-assay <10% CV Inter-assay <15% CV
  • IL-8: Intra-assay <10% CV Inter-assay <15% CV
  • Insulin: Intra-assay <10% CV Inter-assay <15% CV
  • Leptin: Intra-assay <10% CV Inter-assay <15% CV
  • MCP-1: Intra-assay <10% CV Inter-assay <15% CV
  • NGF: Intra-assay <10% CV Inter-assay <15%
  • TNFα: Intra-assay <10% CV Inter-assay <20% CV
Panel TypeMAGNETIC Metabolism
Quality LevelMQ200
Applications
ApplicationThe Human Adipokine Magnetic Bead Panel 2, using the Luminex xMAP technology, enables the simultaneous analysis of nine protein biomarkers in human serum and plasma samples.
Key Applications
  • Multiplexing
Application NotesFor optimal sensitivity, use an overnight (16-18 hour) incubation. Serum and plasma samples do not require a dilution. Each well requires 25 μL of sample, standard or control.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • IL-1β
  • IL-6
  • IL-8
  • Insulin
  • Leptin
  • MCP-1
  • NGF
  • TNF-α
Assay PanelEverything you need in a single kit.
Cross ReactivityEach antibody pair in a panel is highly specific and displays insignificant cross-reactivity to other analytes in the panel.
Accuracy
  • IL-1β 97%
  • IL-6 87%
  • IL-8 87%
  • Insulin 94%
  • Leptin 96%
  • MCP-1 101%
  • NGF 107%
  • TNFα 91%
Physicochemical Information
Sensitivity
  • See kit protocol for individual sensitives.
Linearity of DilutionContact technical support for this information
Standard Curve Range
  • IL-1β: 1.3 - 20,000 pg/mL
  • IL-6: 0.96 - 15,000 pg/mL
  • IL-8: 0.64 - 10,000 pg/mL
  • Insulin: 9.6 - 150,000 pg/mL
  • Leptin: 38 - 600,000 pg/mL
  • MCP-1: 1.3 - 20,000 pg/mL
  • NGF: 0.64 - 10,000 pg/mL
  • TNF-α: 0.64 - 10,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Human Adipokine Magnetic Bead Panel 2

MILLIPLEX MAP Human Adipokine Magnetic Bead Panel 2 - Endocrine Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
Waist-to-height ratio, waist circumference and BMI as indicators of percentage fat mass and cardiometabolic risk factors in children aged 3-7 years.
Sijtsma, A; Bocca, G; L'abée, C; Liem, ET; Sauer, PJ; Corpeleijn, E
Clinical nutrition (Edinburgh, Scotland)  33  311-5  2014

Show Abstract
23768783 23768783
Altered promoter methylation of PDK4, IL1 B, IL6, and TNF after Roux-en Y gastric bypass.
Kirchner, H; Nylen, C; Laber, S; Barrès, R; Yan, J; Krook, A; Zierath, JR; Näslund, E
Surgery for obesity and related diseases : official journal of the American Society for Bariatric Surgery  2014

Show Abstract
24837562 24837562
Insulin resistance is associated with Fibroblast Growth Factor-23 in stage 3-5 chronic kidney disease patients.
Garland, JS; Holden, RM; Ross, R; Adams, MA; Nolan, RL; Hopman, WM; Morton, AR
Journal of diabetes and its complications  28  61-5  2014

Show Abstract
24125760 24125760
Growth differentiation factor 15 and cardiovascular events in patients with stable ischemic heart disease (The Heart and Soul Study).
Schopfer, DW; Ku, IA; Regan, M; Whooley, MA
American heart journal  167  186-192.e1  2014

Show Abstract
24439979 24439979
Racial differences in HbA1c: a cross-sectional analysis of a Brazilian public primary care population.
de Miranda, VA; Cruz Filho, RA; de Oliveira, TS; Moscavitch, SD; Kang, HC; Miranda Chagas, SV; Costa, DM; Vianna Araújo, D; Garcia Rosa, ML
Primary care diabetes  7  135-41  2013

Show Abstract
23485345 23485345

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
Custom Immunoassay Menu of Services
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
HADK2MAG-61K xPonent Protocol Template Version 3.1
HADK2MAG-61K xPonent Protocol Template Version Magpix
Quality Control Ranges: Milliplex® Human Adipokine Magnetic Bead Panel 2

Data Sheet

Title
Full Service Custom-built Assays (Merck)

Posters

Title
MILLIPLEX MAP: Exercise, Signaling, and Your Brain