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HANG2MAG-12K MILLIPLEX® MAP Human Angiogenesis Panel 2, HANG2MAG-12K

HANG2MAG-12K
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Overview

Replacement Information
Description
Catalogue NumberHANG2MAG-12K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® MAP Human Angiogenesis Panel 2, HANG2MAG-12K
OverviewMILLIPLEX® MAP Human Angiogenesis Magnetic Bead Panel 2 contains all the components necessary to measure up to 20 biomarkers in any combination using Luminex® xMAP® technology. This kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls, and can measure up to 38 serum, plasma or cell/tissuue culture samples in duplicate.
Background InformationAngiogenesis, the development of new vascular networks, is a key process in normal growth and development as well as in wound healing, with homeostasis maintained by a delicate balance of angiogenic factors and inhibitors. Consequently, insufficient or excessive blood vessel growth underlies many diseases, including cardiovascular disease, diabetic ulcers, macular degeneration and cancer. Angiogenesis plays a significant role in tumor growth and metastasis.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < XX
  • Intra-Assay: < XX
Panel TypeMAGNETIC Circulating Cancer
Quality LevelMQ200
Applications
ApplicationAngiogenesis Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple angiogenic biomarkers in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight assay requiring 25 µL of 1:5 diluted plasma or serum samples, or 25 µL cell culture supernatant per well.
• This kit may be used for the analysis of all or any combination of the Human Angiogenesis Panel 2 analytes in tissue/cell lysate and culture supernatant or in serum and plasma.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • Angiostatin
  • AXL (soluble)/sAXL
  • c-Kit (soluble) / sc-Kit / sSCFR
  • E-Selectin (soluble) / sE-Selectin
  • EGFR (soluble) / sEGFR / sErbB1 / sHER1
  • HER2 (soluble) / sHER2 / sErbB2
  • HER3 (soluble) / sHER3 / sErbB3
  • HGFR (soluble) / sHGFR / sc-Met
  • Tenascin C / TN-C
  • Thrombospondin-2 / TSP-2
  • IL-6Rα (soluble) / sIL-6Rα
  • Neuropilin-1 (soluble) / sNRP-1
  • TIE-2 (soluble) / sTIE-2
  • uPAR (soluble) / suPAR
  • VEGFR1 (soluble) / sVEGFR1 / sFlt-1
  • VEGFR2 (soluble) / sVEGFR2 / sKDR / sFlk-1
  • VEGFR3 (soluble) / sVEGFR3 / sFlt-4
  • Osteopontin (OPN)
  • PDGF-AB/BB
  • PECAM-1 (soluble) / sPECAM-1 / sCD31
Assay PanelEverything you need in a single kit.
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • 88% - 97%
Physicochemical Information
Sensitivity
  • See kit protocol for individual analytes sensitivities.
Linearity of DilutionContact technical support for this information
Standard Curve Range
  • Angiostatin: 274 – 200,000 pg/mL
  • sAXL: 14 – 10,000 pg/mL
  • sc-Kit/sSCFR: 41 – 30,000 pg/mL
  • sE-Selectin: 274 – 200,000 pg/mL
  • sEGFR: 41 – 30,000 pg/mL
  • sHER2: 41 – 30,000 pg/mL
  • sHER3: 14 – 10,000 pg/mL
  • sHGFR: 69 – 50,000 pg/mL
  • sIL-6Rα: 27 – 20,000 pg/mL
  • sNeuropilin-1: 274 – 200,000 pg/mL
  • Osteopontin (OPN): 41 – 30,000 pg/mL
  • PDGF-AB/BB: 7 – 5,000 pg/mL
  • sPECAM-1: 27 – 20,000 pg/mL
  • Tenascin C: 27 – 20,000 pg/mL
  • Thrombospondin-2: 137 – 100,000 pg/mL
  • sTIE-2: 27 – 20,000 pg/mL
  • suPAR: 137 – 100,000 pg/mL
  • sVEGFR1: 14 – 10,000 pg/mL
  • sVEGFR2: 137 – 100,000 pg/mL
  • sVEGFR3: 412 – 300,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

MILLIPLEX® MAP Human Angiogenesis Panel 2, HANG2MAG-12K SDS

Title

Safety Data Sheet (SDS) 

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Quality Control Ranges: MILLIPLEX® MAP Human Angiogenesis Magnetic Bead Panel 2

Posters

Title
MILLIPLEX MAP: Exercise, Signaling, and Your Brain
Utility of human and mouse multiplex immunoassay panels for studying profiles of circulating angiogenic factors as biomarkers for angiogenesis research (MM)

User Guides

Title
Protocol: Human Angiogenesis Magnetic Bead Panel 2