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HCCBP2MAG-58K MILLIPLEX MAP Human Circulating Cancer Biomarker Magnetic Bead Panel 2 - Cancer Multiplex Assay

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Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Antithrombin III, Complement Factor H, Extracellular Matrix Protein 1, Vitamin D Binding ProteinHMplexLuminex xMAP
Catalogue NumberHCCBP2MAG-58K
Trade Name
DescriptionMILLIPLEX MAP Human Circulating Cancer Biomarker Magnetic Bead Panel 2 - Cancer Multiplex Assay
OverviewMILLIPLEXMAP Circulating Cancer Biomarker Magnetic Bead Panel 2 consists of 4 circulating cancer biomarkers that can be used to study a wide range of tumor types.
Background InformationCancer research has always focused on intracellular biomarkers, such as HER2 and B-Raf, and in recent years, more and more attention has shifted to include extracellular cancer biomarkers. While the use of intracellular markers in research helps clarify the process of oncogenesis, circulating cancer biomarkers give insight into how the body responds to the presence of a tumor, the dysregulation of homeostasis and the relationship between a tumor and its environment. Study of isolated biomarkers, whether intracellular or circulating, is often inadequate to fully characterize the complex relationship between tumor and non-tumor. Consequently, multianalyte panels have become essential tools that enable researchers to measure cancer biomarkers simultaneously, teasing out these relationships and applying them to understand tumor biology.
Product Information
  • Human Circulating Cancer Biomarker Panel 2 Standard (HCC-8058-2)
  • Human Circulating Cancer Biomarker Panel 2 Quality Controls 1 and 2 (HCC-6058-2)
  • Set of one 96-Well Plate with 2 Sealers
  • Bead Diluent (LBD-4)
  • Assay Buffer (L-AB)
    Note: Contains 0.08% Sodium Azide
  • 10X Wash Buffer (L-WB)
    Note: Contains 0.05% Proclin
  • Human Circulating Cancer Biomarker Panel 2 Detection Antibodies (HCC-1058)
  • Streptavidin-Phycoerythrin (L-SAPE 6)
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra Assay: 6 - 9
  • Inter Assay: 7 - 14
Panel TypeMAGNETIC Circulating Cancer
Quality LevelMQ200
ApplicationCirculating Cancer Biomarker Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple oncology biomarkers in various tumor types in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight incubation assay requiring 25 µL of 1:10,000 dilution plasma or serum, or 25 μL of diluted tissue/cell lysate or culture supernatant per well (dilution factors may vary).
• This kit may be used for the analysis of all or any combination of the cancer biomarkers in serum, plasma or tissue/cell lysate and culture. However:
o Serum has a thrombin/antithrombin III complex bound to vitronectin.
o To accurately quantify antithrombin III concentrations, plasma samples are more appropriate because they lack the vitronectin/thrombin/antithrombin complex, better providing true values.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • Antithrombin III
  • Complement Factor H
  • Extracellular Matrix Protein 1
  • Vitamin D Binding Protein
Assay PanelEverything you need in a single kit.
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
  • 92% - 107%
Physicochemical Information
  • Refer to kit protocol for sensitivities of individual biomarkers.
Standard Curve Range
  • 0.01 - 50 ng/mL: Extracellular matrix protein 1
  • 0.48 - 2,000 ng/mL: All other biomarkers
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information



Protocol: Human Circulating Cancer Biomarker Magnetic Bead Panel 2

MILLIPLEX MAP Human Circulating Cancer Biomarker Magnetic Bead Panel 2 - Cancer Multiplex Assay SDS


Safety Data Sheet (SDS) 


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Technical Info

96 well Template 2 Up
96 well Template Single
HCCBP2MAG-58K xPonent Protocol Template Version 3.1
HCCBP2MAG-58K xPonent Protocol Template Version Magpix
QUALITY CONTROL RANGES: MILLIPLEX® MAP Human Circulating Cancer Biomarker Magnetic Bead Panel 2

Data Sheet

Full Service Custom-built Assays (Merck)