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HCCBP1MAG-58K MILLIPLEX MAP Human Circulating Cancer Biomarker Magnetic Bead Panel - Cancer Multiplex Assay

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Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsMultiplexing Ordering RequirementsDetection Methods
AFP CEA CYFRA21-1 FGF-2 HE4 PSA Total PSA free HGF IL-6 IL-8 Leptin MIF OPN - Osteopontin Prolactin SCF sFas sFasL TGF-α TNF-α TRAIL VEGF CA125 CA15-3 CA19-9 β-HCG HMplexPlease note: Total PSA and Free PSA cannot be plexed together.Luminex xMAP
Catalogue NumberHCCBP1MAG-58K
Trade Name
DescriptionMILLIPLEX MAP Human Circulating Cancer Biomarker Magnetic Bead Panel - Cancer Multiplex Assay
OverviewMillipore's MILLIPLEX MAP Circulating Cancer Biomarker magnetic bead panel consists of 25 circulating cancer biomarkers that can be used to study a wide range of tumor types. Some biomarkers are tumor type-specific, such as prostate-specific antigen (PSA), while others, such as IL-6, have been detected in many cancers.

J Alzhimers Dis. 2016 Mar 8;52(2):539-59. doi: 10.3233/JAD-150914.
The Vanderbilt Memory & Aging Project: Study Design and Baseline Cohort Overview.
Jefferson AL, Gifford KA, Acosta LM, Bell SP, Donahue MJ, Davis LT, Gottlieb J, Gupta DK, Hohman TJ, Lane EM, Libon DJ, Mendes LA, Niswender K, Pechman KR, Rane S, Ruberg FL, Su YR, Zetterberg H, Liu D.
Background InformationCancer research has always focused on intracellular biomarkers, such as HER2 and B-Raf, and in recent years, more and more attention has shifted to include soluble cancer biomarkers. While the use of intracellular markers in research helps clarify the process of oncogenesis, circulating cancer biomarkers give insight into how the body responds to the presence of a tumor, the dysregulation of homeostasis and the relationship between a tumor and its environment. Study of isolated biomarkers, whether intracellular or circulating, is often inadequate to fully characterize the complex relationship between tumor and non-tumor. Consequently, multianalyte panels have become essential tools that enable researchers to measure cancer biomarkers simultaneously, teasing out these relationships and applying them to understand tumor biology.
Product Information
  • Human Circulating Cancer Biomarker Panel 1 Standard (HCC-8058)
  • Human Circulating Cancer Biomarker Panel 1 Quality Controls 1 and 2 (HCC-6058)
  • Serum Matrix (HCP-SM)
    Note: Contains 0.08% Sodium Azide
  • Set of one 96-Well Plate with 2 Sealers (MAG-PLATE)
  • Assay Buffer (L-AB)
    Note: Contains 0.08% Sodium Azide
  • 10X Wash Buffer (L-WB)
    Note: Contains 0.05% Proclin
  • Human Circulating Cancer Biomarker Panel 1 Detection Antibodies (HCC-1058 or HCC-1058-2)
  • Streptavidin-Phycoerythrin (L-SAPE 4)
  • Bead Diluent (LBD-4)
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra Assay: 4.9 - 15.0
  • Inter Assay: 4.1 - 16.2
Multiplex Ordering RequirementsPlease note: Total PSA and Free PSA cannot be plexed together.
Panel TypeMAGNETIC Circulating Cancer
Quality LevelMQ200
ApplicationCirculating Cancer Biomarker Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple oncology biomarkers in various tumor types in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight incubation assay requiring 25 µL of 1:6 dilution plasma or serum, or 25 μL cell culture supernatant per well.
• This kit may be used for the analysis of all or any combination of the cancer biomarkers in serum, plasma or tissue/cell lysate and culture supernatant with the following exceptions:
o Total PSA and Free PSA cannot be plexed together
Biological Information
Species Reactivity
  • Human
Analytes Available
  • AFP
  • CEA
  • CYFRA21-1
  • FGF-2
  • HE4
  • PSA Total
  • PSA free
  • HGF
  • IL-6
  • IL-8
  • Leptin
  • MIF
  • OPN - Osteopontin
  • Prolactin
  • SCF
  • sFas
  • sFasL
  • TGF-α
  • TNF-α
  • VEGF
  • CA125
  • CA15-3
  • CA19-9
  • β-HCG
Assay PanelEverything you need in a single kit.
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
  • 35% - 108%
Physicochemical Information
  • Refer to kit protocol for sensitivities of individual biomarkers.
Standard Curve Range
  • 0.09 - 67 mU/mL(β-HCG)
  • 0.7- 500 pg/mL (IL-6)
  • 1.3 - 1000 pg/mL (IL-8, TNFα)
  • 2.7 - 2000 ng/mL (TRAIL, TGF-α)
  • 6.9 - 5000 ng/mL (SCF)
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Global Trade Identification Number
Catalogue Number GTIN



Protocol- HCCBP1MAG-58K

MILLIPLEX MAP Human Circulating Cancer Biomarker Magnetic Bead Panel - Cancer Multiplex Assay SDS


Safety Data Sheet (SDS) 


Reference overviewPub Med ID
A combined blood based gene expression and plasma protein abundance signature for diagnosis of epithelial ovarian cancer--a study of the OVCAD consortium.
Pils, D; Tong, D; Hager, G; Obermayr, E; Aust, S; Heinze, G; Kohl, M; Schuster, E; Wolf, A; Sehouli, J; Braicu, I; Vergote, I; Van Gorp, T; Mahner, S; Concin, N; Speiser, P; Zeillinger, R
BMC cancer  13  178  2013

Show Abstract
23551967 23551967
Effect of different exercise modalities plus a hypocaloric diet on inflammation markers in overweight patients: a randomised trial.
Loria-Kohen, V; Fernández-Fernández, C; Bermejo, LM; Morencos, E; Romero-Moraleda, B; Gómez-Candela, C
Clinical nutrition (Edinburgh, Scotland)  32  511-8  2013

Show Abstract
23177481 23177481


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Technical Info

96 well Template 2 Up
96 well Template Single
HCCBP1MAG-58K xPonent Protocol Template Version Magpix
Quality Control Ranges: Milliplex Human Circulating Cancer Biomarker Panel 1 Magnetic Bead Kit

Data Sheet

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Newsletters / Publications

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