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HSTCMAG-28SK MILLIPLEX MAP Human High Sensitivity T Cell Panel - Immunology Multiplex Assay

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Replacement Information
Replacement InformationMILLIPLEX MAP Human High Sensitivity T Cell Panel, HSTCMAG-28SK replaces MILLIPLEX MAP High Sensitivity Human Cytokine Magnetic Bead Panel, HSCYTMAG-60SK

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Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Fractalkine GM-CSF IFNγ IL-1β IL-2 IL-4 IL-5 IL-6 IL-7 IL-8 IL-10 IL-12 (p70) IL-13 IL-17A IL-21 IL-23 ITAC MIP-1α MIP-1β MIP-3α TNF-α HMplexLuminex xMAP
Catalogue NumberHSTCMAG-28SK
Trade Name
DescriptionMILLIPLEX MAP Human High Sensitivity T Cell Panel - Immunology Multiplex Assay
Brain Behav Immun. 2016 Oct;57:38-46. doi: 10.1016/j.bbi.2016.04.001.
Why sickness hurts: A central mechanism for pain induced by peripheral inflammation.
Karshikoff B, Jensen KB, Kosek E, Kalpouzos G, Soop A, Ingvar M, Olgart Höglund C, Lekander M, Axelsson J.
Background InformationCytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.

Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: <15%
  • Intra-assay: <10%
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
ApplicationSimultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight incubation.
• Because there are unique features concerning sample prep and assay set-up, please read the entire protocol before running the assay. These features are summarized on the next page.
• This kit may be used for the analysis of all or any combination of the Human High Sensitivity T Cell panel analytes in serum, plasma or tissue/cell lysate and culture supernatant.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • Fractalkine
  • GM-CSF
  • IFNγ
  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-8
  • IL-10
  • IL-12 (p70)
  • IL-13
  • IL-17A
  • IL-21
  • IL-23
  • ITAC
  • MIP-1α
  • MIP-1β
  • MIP-3α
  • TNF-α
Cross ReactivityThere is no detectable cross-reactivity within the panel.
  • 98% – 107%
Physicochemical Information
  • 0.11 - 8.17 pg/mL (Overnight Incubation minDC)
  • Please see kit protocol for individual assay sensitivities.
Linearity of DilutionPlease contact technical service for information
Standard Curve Range
  • Fractalkine: 18.3 – 75,000 pg/mL
  • GM-CSF: 1.22 – 5,000 pg/mL
  • IFNγ: 0.61 – 2,500 pg/mL
  • IL-1β: 0.49 – 2,000 pg/mL
  • IL-2: 0.49 – 2,000 pg/mL
  • IL-4: 1.83 – 7,500 pg/mL
  • IL-5: 0.49 – 2,000 pg/mL
  • IL-6: 0.18 – 750 pg/mL
  • IL-7: 0.37 – 1,500 pg/mL
  • IL-8: 0.31 – 1,250 pg/mL
  • IL-10: 1.46 – 6,000 pg/mL
  • IL-12 (p70): 0.49 – 2,000 pg/mL
  • IL-13: 0.24 – 1,000 pg/mL
  • IL-17A: 0.73 – 3,000 pg/mL
  • IL-21: 0.24 – 1,000 pg/mL
  • IL-23: 7.93 – 32,500 pg/mL
  • ITAC: 1.46 – 6,000 pg/mL
  • MIP-1α: 0.31 – 1,250 pg/mL
  • MIP-1β: 0.92 – 3,750 pg/mL
  • MIP-3α: 0.61 – 2,500 pg/mL
  • TNFα: 0.45 – 1,750 pg/mL
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Global Trade Item Number
Catalogue Number GTIN
HSTCMAG-28SK 04053252987663




MILLIPLEX MAP Human High Sensitivity T Cell Panel - Immunology Multiplex Assay SDS


Safety Data Sheet (SDS) 


Reference overviewPub Med ID
Cruciferous vegetable intake is inversely correlated with circulating levels of proinflammatory markers in women.
Jiang, Y; Wu, SH; Shu, XO; Xiang, YB; Ji, BT; Milne, GL; Cai, Q; Zhang, X; Gao, YT; Zheng, W; Yang, G
Journal of the Academy of Nutrition and Dietetics  114  700-8.e2  2014

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Technical Info

96 well Template 2 Up
96 well Template Single
HSTCMAG-28SK xPonent Protocol Template Version 3.1
HSTCMAG-28SK xPonent Protocol Template Version Magpix
HSTCMAG-28SK xPonent Standard Template Version 3.1
HSTCMAG-28SK xPonent Standard Template Version Magpix
MILLIPLEX MAP Human High Sensitivity T Cell Panel
Quality Control Range Sheet- HST-110 and HST-210
Quality Control Ranges: Milliplex Human High Sensitivity T Cell Magnetic Bead Panel

Data Sheet

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