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HMMP1MAG-55K MILLIPLEX MAP Human MMP Magnetic Bead Panel 1 - Immunology Multiplex Assay

HMMP1MAG-55K
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Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
MMP-3 MMP-12 MMP-13 HMplexLuminex xMAP
Description
Catalogue NumberHMMP1MAG-55K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human MMP Magnetic Bead Panel 1 - Immunology Multiplex Assay
OverviewMILLIPLEX MAP Human MMP Magnetic Bead Panel 1 is a 3 plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum/plasma samples: MMP-3, MMP-12 and MMP-13.
Background InformationThe MMPs (matrix metalloproteinases), a family of zinc proteases responsible for the breakdown of extracellular matrix (ECM), play a key role in normal physiological processes, such as embryonic development and tissue morphogenesis, tissue and bone remodeling, wound healing and angiogenesis. These processes rely on MMPs’ role in the cleavage of cell surface receptors, the release of apoptotic ligands, cell proliferation and differentiation, and chemokine activity modulation. Similar in structure, MMPs are synthesized and secreted as inactive pro-enzymes that require proteolytic cleavage for activation. This process can be mediated by serine proteases or other MMPs. An increase in MMP expression occurs in response to a wide range of stimuli, including adhesion molecules, growth factors, cytokines and hormones. Regulation of MMP activity is controlled primarily by TIMPs (tissue inhibitors of metalloproteinases). Therefore, disruption of the MMP/TIMP balance can result in arthritis, cardiovascular disease and tumor growth and metastasis.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay:< 5
  • Inter-assay:< 10
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationMatrix Metalloproteinase Bead-Based Multiplex Assays using the Luminex technology enable the simultaneous analysis of multiple MMPs biomarkers in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This panel was optimized when it was converted to the magnetic bead format. The following changes were made to this kit:
o MMP-12 detection antibody
o Standard curve concentrations for MMP-3 and MMP-13
• This is a 2-hour initial incubation assay requiring 25 μL neat human serum and plasma samples. This panel has also been analytically validated in urine, tissue/cell lystate and culture supernatant samples.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • MMP-3
  • MMP-12
  • MMP-13
Physicochemical Information
Sensitivity
  • See protocol for sensitivities of individual analytes
Standard Curve Range
  • MMP-3: 146 - 150,000 pg/mL
  • MMP-12: 98 - 100,000 pg/mL
  • MMP-13: 58 - 60,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

MILLIPLEX MAP Human MMP Magnetic Bead Panel 1 - Immunology Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
Custom Immunoassay Menu of Services
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
HMMP1MAG-55K xPonent Protocol Template Version Magpix
Quality Control Ranges - Human MMP Magnetic Bead Panel 1

Data Sheet

Title
Full Service Custom-built Assays (Merck)

User Guides

Title
Protocol: Human MMP Panel 1 Magnetic Bead Kit