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HMHEMAG-34K MILLIPLEX MAP Human Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay

The Human Metabolic Hormone Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 13 protein biomarkers in human serum, plasma and cell/tissue culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Metabolism Key Applications: Multiplexing Detection Methods: Luminex xMAP
HMHEMAG-34K
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Overview

Replacement Information
Replacement InformationHuman Metabolic Hormone Magnetic Bead Panel (HMHMAG-34K).

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsMultiplexing Ordering RequirementsDetection Methods
Amylin (Active), Amylin (Total), C-Peptide, Ghrelin (Active), GIP, GLP-1 (Active), GLP-1 (Total), Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY, TNF-αHMplexPlease note: Amylin (Active) and Amylin (Total) cannot be plexed together. GLP-1 (Active) and GLP-1 (Total) cannot be plexed together.Luminex xMAP
Description
Catalogue NumberHMHEMAG-34K
ReplacesHMHMAG-34K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay
OverviewThe MILLIPLEX® MAP Human Metabolic Hormone Magnetic Bead Panel is a 15 analyte, 13-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and cell/tissue culture samples: Amylin (Active) or Amylin (Total), C-Peptide, Ghrelin (Active), GIP, GLP-1 (Active) or GLP-1 (Total), Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY, TNFα.
Background InformationMetabolic syndrome is a cluster of conditions that occur together, including increased blood pressure, elevated insulin levels, excess body fat around the waist, and abnormal cholesterol levels.

Key features of metabolic syndrome include insulin resistance, glucose intolerance, hypertension, dyslipidemia, and central obesity—all of which are risk factors for atherosclerosis, coronary heart disease, type 2 diabetes, kidney disease, and even premature death.

Adults with metabolic syndrome present a low-grade inflammation, whose link with obesity may be a production of pro- and anti-inflammatory factors. Consequently, research done in this area covers multi-faceted fields of cytokines, acute phase proteins, diabetes and obesity related hormones, as well as other cardiovascular disease biomarkers.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Amylin (Active): Intra-assay <10% CV Inter-assay <20%
  • Amylin (Total): Intra-assay <10% CV Inter-assay <20% CV
  • C-Peptide: Intra-assay <10% CV Inter-assay <15% CV
  • Ghrelin (Active): Intra-assay <10% CV Inter-assay <15% CV
  • GIP: Intra-assay <10% CV Inter-assay <15% CV
  • GLP-1 (Active): Intra-assay <10% CV Inter-assay <15% CV
  • GLP-1 (Total): Intra-assay <10% CV Inter-assay <15% CV
  • Glucagon: Intra-assay <10% CV Inter-assay <15% CV
  • IL-6: Intra-assay <10% CV Inter-assay <15% CV
  • Insulin: Intra-assay <10% CV Inter-assay <15% CV
  • Leptin: Intra-assay <10% CV Inter-assay <15% CV
  • MCP-1: Intra-assay <10% CV Inter-assay <15% CV
  • PP: Intra-assay <10% CV Inter-assay <15% CV
  • PYY: Intra-assay <10% CV Inter-assay <15% CV
  • TNFα: Intra-assay <10% CV Inter-assay <15% CV
Multiplex Ordering RequirementsPlease note: Amylin (Active) and Amylin (Total) cannot be plexed together. GLP-1 (Active) and GLP-1 (Total) cannot be plexed together.
Panel TypeMAGNETIC Metabolism
Quality LevelMQ200
Applications
ApplicationThe Human Metabolic Hormone Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 13 protein biomarkers in human serum, plasma and cell/tissue culture samples.
Key Applications
  • Multiplexing
Application Notes• There are 15 biomarkers to choose from in this kit. However, this is a 13-plex kit since the following analytes cannot be run simultaneously: Amylin (Active) & Amylin (Total); GLP-1 (Active) & GLP-1 (Total).
• The following analytes require the addition of protease inhibitors to the serum or plasma samples (see sample collection and storage section in the protocol for details): GLP-1 (DPPIV inhibitor); Amylin (protease inhibitor cocktail); Glucagon (Aprotinin); Ghrelin (Active) (serine protease inhibitor).
• The protocol recommends serum or plasma samples be run neat. Tissue culture samples may be run neat or may require a dilution with an appropriate control medium.
• For optimal sensitivity, use an overnight (16 - 18 hour) incubation.
• Each well requires 25 uL of sample, standard or control.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • Amylin (Active)
  • Amylin (Total)
  • C-Peptide
  • Ghrelin (Active)
  • GIP
  • GLP-1 (Active)
  • GLP-1 (Total)
  • Glucagon
  • IL-6
  • Insulin
  • Leptin
  • MCP-1
  • PP
  • PYY
  • TNF-α
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Accuracy
  • Amylin (Active) 97%
  • Amylin (Total) 104%
  • C-Peptide 99%
  • Ghrelin (Active) 105%
  • GIP 103%
  • GLP-1 (Active) 99%
  • GLP-1 (Total) 103%
  • Glucagon 101%
  • IL-6 94%
  • Insulin 97%
  • Leptin 102%
  • MCP-1 106%
  • PP 104%
  • PYY 107%
  • TNFα 97%
Physicochemical Information
Sensitivity
  • MinDC+2SD
  • Amylin (Active): 28 pg/mL
  • Amylin (Total): 15 pg/mL
  • C-Peptide: 16 pg/mL
  • Ghrelin (Active): 14 pg/mL
  • GIP: 1.1 pg/mL
  • GLP-1 (Active): 2.4 pg/mL
  • GLP-1 (Total): 7.3 pg/mL
  • Glucagon: 16 pg/mL
  • IL-6: 13 pg/mL
  • Insulin: 160 pg/mL
  • Leptin: 64 pg/mL
  • MCP-1: 14 pg/mL
  • PP: 2.9 pg/mL
  • PYY: 146 pg/mL
  • TNFα: 0.5 pg/mL
Linearity of DilutionContact technical support for this information
Standard Curve Range
  • Amylin (Active): Approximately 13.7 - 10,000 pg/mL (Refer to QC analysis sheet for exact concentration.)
  • Amylin (Total): Approximately 13.7 - 10,000 pg/mL (Refer to QC analysis sheet for exact concentration.)
  • C-Peptide: 27.4 - 20,000 pg/mL
  • Ghrelin (Active): 13.7 - 10,000 pg/mL
  • GIP: 1.4 - 1,000 pg/mL
  • GLP-1 (Active): 2.7 - 2,000 pg/mL
  • GLP-1 (Total): 2.7 - 2,000 pg/mL
  • Glucagon: Approximately 13.7 - 10,000 pg/mL (Refer to QC analysis sheet for exact concentration.)
  • IL-6: 13.7 - 10,000 pg/mL
  • Insulin: 137 - 100,000 pg/mL
  • Leptin: 137 - 100,000 pg/mL
  • MCP-1: 13.7 - 10,000 pg/mL
  • PP: 2.7 - 2,000 pg/mL
  • PYY: 13.7 - 10,000 pg/mL
  • TNFα: 0.69 - 500 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Human Metabolic Hormone Magnetic Bead Panel

MILLIPLEX MAP Human Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
Custom Immunoassay Menu of Services
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
HMHEMAG-34K xPonent Protocol Template Version Magpix
HMHEMAG-34K xPonent Standard Template Version Magpix
MILLIPLEX® MAP metabolic hormones in human serum and plasma
QUALITY CONTROL RANGES Milliplex Human Metabolic Hormone Magnetic Bead Panel

Data Sheet

Title
Full Service Custom-built Assays (Merck)