Millipore Sigma Vibrant Logo

HSP3MAG-63K MILLIPLEX MAP Human Sepsis Magnetic Bead Panel 3 - Immune Response Multiplex Assay

HSP3MAG-63K
Design And Price Your Kit

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Lactoferrin, Lipocalin-2/NGAL, Neutrophil Elastase-2 (ELA2), Resistin, Thrombospondin-1 (TSP-1)HMplexLuminex xMAP
Description
Catalogue NumberHSP3MAG-63K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Sepsis Magnetic Bead Panel 3 - Immune Response Multiplex Assay
Background InformationSepsis or SIRS (systemic inflammatory response syndrome) results from infection, whether bacterial, viral, fungal or parasitic. According to the CDC, sepsis is the second leading cause of death in non-coronary ICU patients in the U.S. The pathogenesis of sepsis is very complex. Both an innate and acquired immune response to infection interrupts homeostasis with a shift of cytokine expression that increases immunosuppression. In addition migration of the leukocytes in the inflamed tissues secretes a tissue factor leading to the formation of thrombin, also triggering the release of chemokines and adhesion molecules through endothelial cells. Severe sepsis occurs when hypotension or hypoperfusion to one or more organs leads to organ dysfunction, which, in turn, can cause septic shock, multiple organ dysfunction syndrome (MODS) and death.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 15
  • Intra-assay: < 10
Panel TypeMAGNETIC Immune Response
Quality LevelMQ200
Applications
ApplicationInflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple sepsis biomarkers in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is a 2-hour incubation assay.
• This assay requires 25 µL of a 1:100 dilution of plasma or serum, or 25 μL cell culture supernatant per well.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • Lactoferrin
  • Lipocalin-2/NGAL
  • Neutrophil Elastase-2 (ELA2)
  • Resistin
  • Thrombospondin-1 (TSP-1)
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • Lactoferrin 82%
  • Lipocalin-2/NGAL 98%
  • Neutrophil Elastase-2 (ELA2) 89%
  • Resistin 93%
  • Thrombospondin-1 (TSP1) 95%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for individual sensitivites.
Standard Curve Range
  • Lactoferrin and Thrombospondin-1 (TSP1): 195 - 200,000 pg/mL
  • Lipocalcin-2/NGAL: 29 - 30,000 pg/mL
  • Neutrophil Elastase-2 (ELA2): 20 - 20,000 pg/mL
  • Resistin: 10 - 10,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Human Sepsis Panel 3 Magnetic Bead Panel

MILLIPLEX MAP Human Sepsis Magnetic Bead Panel 3 - Immune Response Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
Custom Immunoassay Menu of Services
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Quality Control Ranges - Milliplex Human Sepsis Magnetic Bead Panel 3

Data Sheet

Title
Full Service Custom-built Assays (Merck)

Posters

Title
Inflammation in Neurodegenerative Diseases
MILLIPLEX® MAP Cell Signaling Wall Chart (MM)