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MCYTOMAG-70K MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay

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Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
G-CSF GM-CSF IFN-γ IL-1α IL-1β IL-2 IL-3 IL-4 IL-5 IL-6 IL-7 IL-9 IL-10 IL-12 (p40) IL-12 (p70) IL-13 IL-15 IL-17 IP-10 KC LIF LIX MCP-1 M-CSF MIG MIP-1α MIP-1β MIP-2 RANTES TNF-α VEGF Eotaxin/CCL11 MMplexLuminex xMAP
Catalogue NumberMCYTOMAG-70K
Trade Name
DescriptionMILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay
OverviewTo identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:
• More flexible plate and plate washer options
• Improved performance with turbid serum/plasma samples
• Assay results equivalent to non-magnetic beads
• Automated magnetic washing avoids many problems associated with vacuum filtration washing

MILLIPLEX MAP Mouse Cytokine / Chemokine panel enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.

Neuroscience. 2015 Sep 24;304:316-27. doi: 10.1016/j.neuroscience.2015.07.070.
Nilotinib and bosutinib modulate pre-plaque alterations of blood immune markers and neuro-inflammation in Alzheimer's disease models.
Lonskaya I, Hebron ML, Selby ST, Turner RS, Moussa CE.

Mol Neurodegener. 2016 Apr 2;11:25. doi: 10.1186/s13024-016-0090-7.
Pioglitazone ameliorates the phenotype of a novel Parkinson's disease mouse model by reducing neuroinflammation.
Pinto M, Nissanka N, Peralta S, Brambilla R, Diaz F, Moraes CT.

Sci Rep. 2016 May 13;6:25663. doi: 10.1038/srep25663.
CSF1R blockade slows the progression of amyotrophic lateral sclerosis by reducing microgliosis and invasion of macrophages into peripheral nerves.
Martínez-Muriana A, Mancuso R, Francos-Quijorna I, Olmos-Alonso A, Osta R, Perry VH, Navarro X, Gomez-Nicola D, López-Vales R.

Neuropharmacology. 2016 Apr 20. pii: S0028-3908(16)30161-7. doi: 10.1016/j.neuropharm.2016.04.021.
Ketamine modulates hippocampal neurogenesis and pro-inflammatory cytokines but not stressor induced neurochemical changes.
Clarke M, Razmjou S, Prowse N, Dwyer Z, Litteljohn D, Pentz R, Anisman H, Hayley S.

Front Cell Neurosci. 2016 Jun 28;10:169. doi: 10.3389/fncel.2016.00169. eCollection 2016.
Allergy Enhances Neurogenesis and Modulates Microglial Activation in the Hippocampus.
Klein B, Mrowetz H, Thalhamer J, Scheiblhofer S, Weiss R, Aigner L.

Glia. 2016 Sep;64(9):1479-91. doi: 10.1002/glia.23016.
Microglial phagocytosis and activation underlying photoreceptor degeneration is regulated by CX3CL1-CX3CR1 signaling in a mouse model of retinitis pigmentosa.
Zabel MK, Zhao L, Zhang Y, Gonzalez SR, Ma W, Wang X, Fariss RN, Wong WT.

J Immunol. 2016 Apr 15;196(8):3318-30. doi: 10.4049/jimmunol.1502021.
Age-Associated Resident Memory CD8 T Cells in the Central Nervous System Are Primed To Potentiate Inflammation after Ischemic Brain Injury.
Ritzel RM, Crapser J, Patel AR, Verma R, Grenier JM, Chauhan A, Jellison ER, McCullough LD.

Mol Psychiatry. 2016 Jul;21(7):956-68. doi: 10.1038/mp.2015.222.
Maternal immune activation leads to selective functional deficits in offspring parvalbumin interneurons.
Canetta S, Bolkan S, Padilla-Coreano N, Song LJ, Sahn R, Harrison NL, Gordon JA, Brown A, Kellendonk C.

J Mol Cell Cardiol. 2017 Jun;107:22-26. doi: 10.1016/j.yjmcc.2017.04.003.
A humanized HLA-DR4 mouse model for autoimmune myocarditis.
Selli ME, Thomas AC, Wraith DC, Newby AC.
Background Information The immune system is an organized complex network of biological structures and processes that protect against disease. For example, cytokine and chemokines mediate interactions between cells directly, regulating target immune cell responses. These responses result in inflammation where the immune system attempts to eradicate foreign antigens and begin the healing process. Consequently, the inflammatory process plays a key protective role in immunity. In addition cytokine and chemokine research is essential in understanding the immune system and its multi-faceted response to most antigens, as well as disease states such as autoimmune disease, allergic reactions, sepsis and cancer.
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: 4 - 12
  • Intra-assay: 1 - 5
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
ApplicationSimultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.
• This assay requires 25 µl of 1:2 diluted plasma or serum, or 25 μL cell culture supernatant per well.
Biological Information
Species Reactivity
  • Mouse
Analytes Available
  • G-CSF
  • GM-CSF
  • IFN-γ
  • IL-1α
  • IL-1β
  • IL-2
  • IL-3
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-9
  • IL-10
  • IL-12 (p40)
  • IL-12 (p70)
  • IL-13
  • IL-15
  • IL-17
  • IP-10
  • KC
  • LIF
  • LIX
  • MCP-1
  • M-CSF
  • MIG
  • MIP-1α
  • MIP-1β
  • MIP-2
  • TNF-α
  • VEGF
  • Eotaxin/CCL11
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
  • 85% - 107%
Physicochemical Information
  • Refer to kit protocol for sensitivities for individual cytokines.
Standard Curve Range
  • 3.2 – 10,000 pg/mL
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information




MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay SDS


Safety Data Sheet (SDS) 


Reference overviewPub Med ID
Intestinal inflammation in a murine model of autism spectrum disorders.
de Theije, CG; Koelink, PJ; Korte-Bouws, GA; Lopes da Silva, S; Korte, SM; Olivier, B; Garssen, J; Kraneveld, AD
Brain, behavior, and immunity  37  240-7  2014

Show Abstract
24321212 24321212
Lipopolysaccharide-induced brain activation of the indoleamine 2,3-dioxygenase and depressive-like behavior are impaired in a mouse model of metabolic syndrome.
Dinel, AL; André, C; Aubert, A; Ferreira, G; Layé, S; Castanon, N
Psychoneuroendocrinology  40  48-59  2014

Show Abstract
24485475 24485475
Local hyperthermia treatment of tumors induces CD8(+) T cell-mediated resistance against distal and secondary tumors.
Toraya-Brown, S; Sheen, MR; Zhang, P; Chen, L; Baird, JR; Demidenko, E; Turk, MJ; Hoopes, PJ; Conejo-Garcia, JR; Fiering, S
Nanomedicine : nanotechnology, biology, and medicine  10  1273-85  2014

Show Abstract
24566274 24566274
Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments.
Sprague, L; Muccioli, M; Pate, M; Singh, M; Xiong, C; Ostermann, A; Niese, B; Li, Y; Li, Y; Courreges, MC; Benencia, F
Experimental cell research  323  7-27  2014

Show Abstract
24569142 24569142
Non-viability of crossing the Alzheimer mouse model Tg2576 with the type 2 diabetes mouse model ob/ob.
Lubitz, I; Haroutunian, V; Katsel, P; Leroith, D; Landa, N; Castel, D; Shaish, A; Shnerb, R; Schnaider-Beeri, M
Neurobiology of aging  35  e19-20  2014

24581479 24581479
Integrated analysis of microRNAs and their disease related targets in the brain of mice infected with West Nile virus.
Kumar, M; Nerurkar, VR
Virology  452-453  143-51  2014

Show Abstract
24606691 24606691
Schizonepeta tenuifolia inhibits the development of atopic dermatitis in mice.
Choi, YY; Kim, MH; Kim, JH; Jung, HS; Sohn, Y; Choi, YJ; Hwang, MK; Kim, SH; Kim, J; Yang, WM
Phytotherapy research : PTR  27  1131-5  2013

Show Abstract
22949410 22949410
Iron oxide nanoparticles as a clinically acceptable delivery platform for a recombinant blood-stage human malaria vaccine.
Pusic, K; Aguilar, Z; McLoughlin, J; Kobuch, S; Xu, H; Tsang, M; Wang, A; Hui, G
FASEB journal : official publication of the Federation of American Societies for Experimental Biology  27  1153-66  2013

Show Abstract
23195035 23195035
Dectin immunoadhesins and pneumocystis pneumonia.
Ricks, DM; Chen, K; Zheng, M; Steele, C; Kolls, JK
Infection and immunity  81  3451-62  2013

Show Abstract
23836814 23836814
Compartmentalization of immune responses during Staphylococcus aureus cranial bone flap infection.
Cheatle, J; Aldrich, A; Thorell, WE; Boska, MD; Kielian, T
The American journal of pathology  183  450-8  2013

Show Abstract
23747950 23747950


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Technical Info

96 well Template 2 Up
96 well Template Single
MCYTOMAG-70K xPonent Protocol Template Version 3.1
MCYTOMAG-70K xPonent Protocol Template Version Magpix
MCYTOMAG-70K xPonent Standard Template Version 3.1
MCYTOMAG-70K xPonent Standard Template Version Magpix
Quality Control Range Sheet- MCC-114 and MCC-214
Quality Control Range Sheet- MCY-111 and MCY2-211
Quality Control Ranges - Milliplex Mouse Cytokine/Chemokine Magnetic Panel
Quality Control Ranges: MILLIPLEX® MAP Mouse Cytokine/Chemokine Panel

Data Sheet

Full Service Custom-built Assays


MILLIPLEX MAP: Exercise, Signaling, and Your Brain

Newsletters / Publications

Reprint: A humanized HLA-DR4 mouse model for autoimmune myocarditis