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MHSTCMAG-70K MILLIPLEX MAP Mouse High Sensitivity T Cell Panel - Immunology Multiplex Assay

Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Sub-Category: Immune Response, Metabolic Disorders, Chronic Inflammatory Disease, Cardiovascular Disease
MHSTCMAG-70K

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Overview

Replacement Information

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Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 (p70), IL-13, IL-17A, KC/CXCL1, LIX, MCP-1, MIP-2, TNF-αMMplexLuminex xMAP
Description
Catalogue NumberMHSTCMAG-70K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Mouse High Sensitivity T Cell Panel - Immunology Multiplex Assay
Background InformationCytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells), and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells, and myeloid cells. Research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.

Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: <15%
  • Intra-assay: <10%
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationSimultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight incubation assay.
• A maximum of 25 μL per well of neat or1:2 diluted serum or plasma can be used. Tissue culture or other media may also be used.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Mouse
Analytes Available
  • GM-CSF
  • IFNγ
  • IL-1α
  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-10
  • IL-12 (p70)
  • IL-13
  • IL-17A
  • KC/CXCL1
  • LIX
  • MCP-1
  • MIP-2
  • TNF-α
Cross ReactivityThere is no detectable cross-reactivity within the panel.
Accuracy
  • 82-102%
Physicochemical Information
Sensitivity
  • 0.26 - 11.14 pg/mL
  • Please see protocol for sensitivity of individual analytes.
Linearity of DilutionPlease contact technical service for information
Standard Curve Range
  • GM-CSF: 6.1 – 25,000 pg/mL
  • IFNγ: 0.49 – 2,000 pg/mL
  • IL-1α: 3.91 – 16,000 pg/mL
  • IL-1β: 3.91 – 16,000 pg/mL
  • IL-2: 0.98 – 4,000 pg/mL
  • IL-4: 0.10 – 400 pg/mL
  • IL-5: 0.98 – 4,000 pg/mL
  • IL-6: 0.98 – 4,000 pg/mL
  • IL-7: 0.49 – 2,000 pg/mL
  • IL-10: 1.34 – 5,500 pg/mL
  • IL-12 (p70): 2.93 – 12,000 pg/mL
  • IL-13: 3.42 – 14.000 pg/mL
  • IL-17A: 0.49 – 2,000 pg/mL
  • KC/GROα/CXCL1: 0.73 – 3,000 pg/mL
  • LIX: 3.17 – 13,000 pg/mL
  • MCP-1/CCL2: 3.91 – 16,000 pg/mL
  • MIP-2/CXCL2: 7.32 – 30,000 pg/mL
  • TNFα: 0.49 – 2,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

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Life Science Research > Protein Detection and Quantification > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Configurable Kits