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MKI1MAG-94K MILLIPLEX MAP Mouse Kidney Injury Magnetic Bead Panel 1 - Toxicity Multiplex Assay

The analytes available for this multiplex kit are: β-2-Microglobulin, IP-10, KIM-1, Renin, TIMP-1, VEGF (for urine samples) or IP-10, KIM-1, Renin, and TIMP-1 (for serum/plasma samples).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Sub-Category: Toxicity Key Applications: Multiplexing Research Category: Toxicity Detection Methods: Luminex xMAP
MKI1MAG-94K
Design And Price Your Kit

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
β-2-Microglobulin, IP-10, KIM-1, Renin, TIMP-1, VEGFMMplexLuminex xMAP
Description
Catalogue NumberMKI1MAG-94K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Mouse Kidney Injury Magnetic Bead Panel 1 - Toxicity Multiplex Assay
OverviewThe MILLIPLEX® MAP Mouse Kidney Injury Magnetic Bead Panel 1 contains all the components necessary to measure the following six biomarkers in any combination using Luminex® xMAP® technology: β-2-Microglobulin, IP-10, KIM-1, Renin, TIMP-1, VEGF. The kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 urine samples in duplicate.
Background InformationMaintenance of physiological balance in the body is attributed in part to normal kidney function. An altered kidney function due to injury, drug toxicity or kidney failure may be life threatening. There are multiple risk factors for kidney injury including age, hypertension, diabetes mellitus, heart failure and liver failure. On the other hand, drug induced damage to the kidney results from drug excretion. Traditional markers for assessing renal toxicity, such as blood urea nitrogen (BUN) and serum creatinine, are insensitive and non-specific. Although both are direct measurements of renal function, increases in serum concentrations of these biomarkers occur only after substantial renal injury. Specificity and earlier detection of kidney injury is vital. For improved detection of acute nephrotoxicity, a panel of novel urinary kidney biomarkers has been identified and validated for preclinical studies in relevant animal species.

We provide valuable research assays to investigate multiple biomarkers of kidney injury in mouse urine samples using the Luminex xMAP® instrument platform.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 10
  • Intra-Assay: < 10
Panel TypeMAGNETIC Toxicity
Quality LevelMQ200
Applications
ApplicationThe analytes available for this multiplex kit are: β-2-Microglobulin, IP-10, KIM-1, Renin, TIMP-1, VEGF (for urine samples) or IP-10, KIM-1, Renin, and TIMP-1 (for serum/plasma samples).
Key Applications
  • Multiplexing
Application Notes• This is an overnight assay.
• This assay requires 25 µL of 1:25 diluted urine sample per well in kit Assay Buffer (please dilute per protocol instructions).
Biological Information
SpecificityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Species Reactivity
  • Mouse
Analytes Available
  • β-2-Microglobulin
  • IP-10
  • KIM-1
  • Renin
  • TIMP-1
  • VEGF
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Accuracy
  • 73% - 98%
Physicochemical Information
Sensitivity
  • 0.001 - 0.041 ng/mL (MinDC+2SD).
  • Please see kit protocol for individual assay sensitivities.
Linearity of DilutionContact technical support for this information.
Standard Curve Range
  • β-2-Microglobulin: 0.05 – 50 ng/mL
  • IP-10: 0.005 – 5 ng/mL
  • KIM-1: 0.01 – 15 ng/mL
  • Renin: 0.05 – 50 ng/mL
  • TIMP-1: 0.02 – 25 ng/mL
  • VEGF: 0.001 – 1 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Mouse Kidney Injury Magnetic Bead Panel 1

MILLIPLEX MAP Mouse Kidney Injury Magnetic Bead Panel 1 - Toxicity Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
Custom Immunoassay Menu of Services
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Quality Control Ranges - Milliplex Mouse Kidney Injury Magnetic Bead Panel 1

Data Sheet

Title
Full Service Custom-built Assays (Merck)

Posters

Title
Novel Kidney Injury Biomarker Panels To Detect Nephrotoxicity in Mouse Models
Toxicology in drug discovery and development: Biomarkers and model organisms