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RCI1MAG-87K MILLIPLEX MAP Rat Cardiac Injury Panel 1 - Cardiotoxicity Multiplex Assay

RCI1MAG-87K
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Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Troponin I (TnI) Troponin T (TnT) Creatine Kinase, Muscle (CKM) FABP3 Follistatin-like Protein 1 (FSTL1) Myosin Light Chain 3 (MYL3) TIMP-1 RMplexLuminex xMAP
Description
Catalogue NumberRCI1MAG-87K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Rat Cardiac Injury Panel 1 - Cardiotoxicity Multiplex Assay
OverviewThe MILLIPLEX® MAP Rat Cardiac Injury Magnetic Bead Panel 1 (RCI1MAG-87K) contains all the components necessary to simultaneous quantify any or all of the following 7 analytes in serum and plasma samples:

• Cardiac Troponin I (cTnI)
• Cardiac Troponin T (cTnT)
• Creatine Kinase, Muscle (CKM)
• Fatty Acid Binding Protein 3 (FABP3)
• Follistatin-like Protein 1 (FSTL1)
• Myosin Light Chain 3 (MYL3)
• Tissue Inhibitor of Metalloproteinase-1 (TIMP-1)

This kit uses a 96-well format, contains lyophilized standard cocktail and two quality controls and can measure up to 38 serum or plasma samples in duplicate.
Background InformationDrug-induced cardiotoxicity can be life threatening and necessitates limiting an otherwise-effective treatment. In fact, cardiovascular toxicity has been reported to account for over 40% of drug candidate withdrawal in premarketing clinical trials.

Many therapeutic agents have been reported to induce or exacerbate heart failure including anti-tumor medications, immunomodulating drugs, antidepressants and calcium channel blocking agents to name a few. Conventional biomarkers of cardiotoxicity such as aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine kinase (CK) have gradually been withdrawn due to low tissue specificity. The number of cardiac-specific biomarkers adapted from clinical studies of cardiotoxicity continues to rise. The reverse translational application of these auspicious biomarkers in experimental animal models could improve the power of detection for diagnosis, helping to predict the occurrence of cardiotoxicity in humans.

Emerging biomarkers of cardiac injury include multiple categories of proteins: characteristic muscle cell proteins (cTnI, cTnT, FABP3 and MYL3), cytosolic kinase (CKM), secreted heparin-binding protein (FSTL1) and metalloprotease inhibitor (TIMP1). Numerous studies have demonstrated elevated levels of these biomarkers in experimental animal blood samples. In order to meet the increasing need to provide screening assays for experimental biomarkers of drug-induced cardiac injury in pre-clinical and translational research models, EMD Millipore has developed the MILLIPLEX® MAP Rat Cardiac Injury Magnetic Bead Panel 1. This confugurable 7-plex panel is the most versatile system available for cardiac injury screening in pre-clinical and translational research models.
References
Product Information
Components
  • Rat Cardiac Injury Standard, 1 vial, lyophilized
  • Rat Cardiac Injury Quality Control 1, 1 vial, lyophilized
  • Rat Cardiac Injury Quality Control 2, 1 vial, lyophilized
  • Serum Matrix (0.08% Sodium Azide), 1 vial, lyophylized
  • Assay Buffer, 1 bottle, 30 mL
  • Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
  • Rat Cardiac Injury Panel 1 Detection Antibodies, 1 bottle, 3.2 mL
  • Rat Cardiac Injury Panel 1 Antibody-Immobilized Beads (20X), 200 µL ea
  • Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL
  • Mixing Bottle, 1 bottle
  • Sample Plate, 96 well format, 1 plate
  • 2 Foil Plate Sealers
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: <15
  • Intra-assay: <10
Panel TypeMAGNETIC Toxicity
Applications
ApplicationThe MILLIPLEX® MAP Rat Cardiac Injury Magnetic Bead Panel 1 is a configurable 7-plex kit for the quantification of cTnI, cTnT, CKM (MM-CK), FABP3, FSTL1, MYL3, and TIMP-1 in serum or plasma samples on the Luminex xMAP® instrument platform.
Key Applications
  • Multiplexing
Application Notes• This assay requires 25 µL of 1:5 diluted serum or plasma sample per well in kit Assay Buffer (please dilute per protocol instructions).
• This assay protocol includes instruction for 2 Hour or Overnight incubation.
Biological Information
SpecificityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Species Reactivity
  • Rat
Analytes Available
  • Troponin I (TnI)
  • Troponin T (TnT)
  • Creatine Kinase, Muscle (CKM)
  • FABP3
  • Follistatin-like Protein 1 (FSTL1)
  • Myosin Light Chain 3 (MYL3)
  • TIMP-1
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Accuracy
  • 2 Hour Protocol: 80% - 100% Recovery
  • Overnight Protocol: 86% - 103% Recovery
  • Please refer to Assay Protocol for individual analyte accuracy.
Physicochemical Information
Sensitivity
  • 2 Hour Protocol: 9 - 86 pg/mL (MinDC+2SD).
  • Overnight Protocol: 7 - 155 pg/mL (MinDC+2SD).
  • Please see kit protocol for assay sensitivities of individual analytes.
Linearity of DilutionContact technical support for this information.
Standard Curve Range
  • cTnI: 123 – 90,000 pg/mL
  • cTnT: 82 – 60,000 pg/mL
  • CKM: 21 – 15,000 pg/mL
  • FABP3: 41 – 30,000 pg/mL
  • FSTL1: 82 – 60,000 pg/mL
  • MYL3: 4 – 3,000 pg/mL
  • TIMP-1: 41 – 30,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Rat Cardiac Injury Magnetic Bead Panel 1

MILLIPLEX MAP Rat Cardiac Injury Panel 1 - Cardiotoxicity Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
Analyte Quarterly Vol. 2 2020
Custom Immunoassay Menu of Services

Technical Info

Title
96 well Template 2 Up
96 well Template Single
QUALITY CONTROL RANGES MILLIPLEX® MAP Rat Cardiac Injury Magnetic Bead Panel 1