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RECYTMAG-65K MILLIPLEX MAP Rat Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay

RECYTMAG-65K

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Overview

Replacement Information
Replacement InformationThis kit replaces RCYTOMAG-80K

Special Offers

This panel is available for custom premix. Talk to your Protein Specialist today!

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
EGF Eotaxin/CCL11 Fractalkine G-CSF GM-CSF GRO/KC IFN-γ IL-1α IL-1β IL-2 IL-4 IL-5 IL-6 IL-10 IL-12 (p70) IL-13 IL-17A IL-18 IP-10 Leptin LIX MCP-1 MIP-1α MIP-2 RANTES TNF-α VEGF RMplexLuminex xMAP
Description
Catalogue NumberRECYTMAG-65K
ReplacesRCYTOMAG-80K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Rat Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay
OverviewThe MILLIPLEX MAP Rat Cytokine/Chemokine Magnetic Bead Panel (RECYTMAG-65K) enables you to focus on the therapeutic potential of cytokines, as well as the modulation of cytokine expression.

References:
Neurosci Lett. 2015 Aug 18;602:120-5. doi: 10.1016/j.neulet.2015.06.040.
Neuroprotective effects of bisperoxovanadium on cerebral ischemia by inflammation inhibition.
Mao LL, Hao DL, Mao XW, Xu YF, Huang TT, Wu BN, Wang LH.

J Cereb Blood Flow Metab. 2016 Mar 16. pii: 0271678X16638669.
Delayed ALK5 inhibition improves functional recovery in neonatal brain injury.
Guardia Clausi M, Levison SW.
Background InformationThe immune system is an organized complex network of biological structures and processes that protect against disease. For example, cytokine and chemokines mediate interactions between cells directly, regulating target immune cell responses. These responses result in inflammation where the immune system attempts to eradicate foreign antigens and begin the healing process. Consequently, the inflammatory process plays a key protective role in immunity. In addition cytokine and chemokine research is essential in understanding the immune system and its multi-faceted response to most antigens, as well as disease states such as autoimmune disease, allergic reactions, sepsis and cancer.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-Assay: <25
  • Intra-Assay: <10
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationSimultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in rat serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application NotesThis is a 2-hour room-temperature incubation assay requiring 25 μL 1:2 dilution rat serum or plasma samples.
Biological Information
Species Reactivity
  • Rat
Analytes Available
  • EGF
  • Eotaxin/CCL11
  • Fractalkine
  • G-CSF
  • GM-CSF
  • GRO/KC
  • IFN-γ
  • IL-1α
  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-10
  • IL-12 (p70)
  • IL-13
  • IL-17A
  • IL-18
  • IP-10
  • Leptin
  • LIX
  • MCP-1
  • MIP-1α
  • MIP-2
  • RANTES
  • TNF-α
  • VEGF
Cross ReactivityThere is no or minimum cross-reactivity between the antibodies and any other analytes in the panel.
Accuracy
  • 80 - 131%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivites for individual cytokines/chemokines
Standard Curve Range
  • EGF: 0.2 – 1,000 pg/mL
  • Eotaxin, C-CSF, IL-4, IL-5, IL-13, RANTES, VEGF: 4.9 – 20,000 pg/mL
  • Fractalkine, IP-10, Leptin, TNFα: 2.4 – 10,000 pg/mL
  • GM-CSF, IL-1α, IL-2, IL-12 (p70), IL-18: 12.2 – 50,000 pg/mL
  • GRO/KC: 1.2 – 5,000 pg/mL
  • IFNγ, MIP-1α: 14.6 – 60,000 pg/mL
  • IL-6: 73.2 – 300,000 pg/mL
  • IL-10, IL-17A, MCP-1: 7.3 – 30,000 pg/mL
  • LIX, MIP-2: 24.4 – 100,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol- RECYTMAG-65K,RECYMAG65K27PMX,RECYMAG65PMX27BK

MILLIPLEX MAP Rat Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Immunoassay Platform Solutions.
Analyte Quarterly Vol. 2 2021
Analyte Quarterly Vol. 2 2021
Custom Immunoassay Menu of Services

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Development of a MILLIPLEX®MAP multiplexed assay panel for emerging biomarkers
Quality Control Range Sheet- RCY- 112 and RCY-212
RECYTMAG-65K xPonent Protocol Template Version 3.1
RECYTMAG-65K xPonent Protocol Template Version Magpix

Data Sheet

Title
Full Service Custom-built Assays

Posters

Title
MILLIPLEX MAP: Exercise, Signaling, and Your Brain
MILLIPLEX®MAP Cell Signaling Wall Chart

Newsletters / Publications

Title
Research article: Analytical performance of a commercial multiplex Luminex-based cytokine panel in the rat