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FCMAB288A6 Milli-Mark™ Anti-Ubiquitin Antibody, Lys63-Specific-Alexa Fluor®647, clone Apu3

This Milli-Mark Anti-Ubiquitin Antibody, Lys63-Specific-Alexa Fluor®647, clone Apu3 is validated for use in FC for the detection of Ubiquitin.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Ubiquitin & Ubiquitin Metabolism UniProt Number: P0CG48
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FCMAB288A6
100 tests  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCRbAlexaFluor®647Monoclonal Antibody
      Description
      Catalogue NumberFCMAB288A6
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark™ Anti-Ubiquitin Antibody, Lys63-Specific-Alexa Fluor®647, clone Apu3
      Background InformationPolyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
      References
      Product Information
      FormatAlexaFluor®647
      Control
      • HeLa cells
      PresentationPurified recombinant rabbit monoclonal IgG1 conjugated to Alexa Fluor 647 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Quality LevelMQ100
      Applications
      ApplicationThis Milli-Mark Anti-Ubiquitin Antibody, Lys63-Specific-Alexa Fluor®647, clone Apu3 is validated for use in FC for the detection of Ubiquitin.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenImmunogen used was di-ubiquitin linked at Lys63.
      EpitopeLys63 ubiquitination site
      CloneApu3
      HostRabbit
      SpecificityRecognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ref.21


      SUBCELLULAR LOCATION: Cytoplasm. Nucleus.


      PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.


      MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).


      SIMILARITY: Belongs to the ubiquitin family.
      Molecular WeightMolecular weight varies.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      FCMAB288A6 04053252680915

      Documentation

      Milli-Mark™ Anti-Ubiquitin Antibody, Lys63-Specific-Alexa Fluor®647, clone Apu3 SDS

      Title

      Safety Data Sheet (SDS) 

      Milli-Mark™ Anti-Ubiquitin Antibody, Lys63-Specific-Alexa Fluor®647, clone Apu3 Certificates of Analysis

      TitleLot Number
      Milli-Mark Anti-Ubiquitin, Lys63- Specific-Alexa Fluor#174;647, clone Apu3 - NRG1817667 NRG1817667
      Milli-Mark™ Anti-Ubiquitin, Lys63- -2780979 2780979
      Milli-Mark™ Anti-Ubiquitin, Lys63-Specific-Alexa Fluor®647, clone Apu3 - 2495478 2495478
      Milli-Mark™ Anti-Ubiquitin, Lys63-Specific-Alexa Fluor®647, clone Apu3 - 2543761 2543761
      Milli-Mark™ Anti-Ubiquitin, Lys63-Specific-Alexa Fluor®647, clone Apu3 - 3764825 3764825
      Milli-Mark™ Anti-Ubiquitin, Lys63-Specific-Alexa Fluor®647, clone Apu3 - 4295878 4295878
      Milli-Mark™ Anti-Ubiquitin, Lys63-Specific-Alexa Fluor®647, clone Apu3 -2650817 2650817
      Milli-Mark™ Anti-Ubiquitin, Lys63-Specific-Alexa Fluor®647, clone Apu3 Conjugate 2951435
      Milli-Mark™ Anti-Ubiquitin, Lys63-Specific-Alexa Fluor®647, clone Apu3 Conjugate 3041058
      Milli-Mark™ Anti-Ubiquitin, Lys63- Specific-Alexa Fluor®647, clone Apu3 - 2204968 2204968
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