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482721 Nitric Oxide Synthase, Constitutive, Rat Neuronal, Recombinant, S. frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: nNOS, Rat, Neuronal Nitric Oxide Synthase

482721
  
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      Recombinant, rat neuronal NOS expressed in S. frugiperda insect cells using a baculovirus expression system.
      Catalogue Number482721
      Brand Family Calbiochem®
      SynonymsnNOS, Rat, Neuronal Nitric Oxide Synthase
      References
      ReferencesAbu-Soud, H.M., et al. 1994. J. Biol. Chem. 269, 32047.
      Abu-Soud, H.M., et al. 1994. J. Biol. Chem. 269, 32318.
      Richards, M.K., and Marletta, M.A. 1994. Biochemistry 33, 14723.
      Sheta, E.A., et al. 1994. J. Biol. Chem. 269, 15147.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will produce 1 nmol of nitric oxide per min in the presence of calmodulin at 37°C, pH 7.5.
      FormLiquid
      FormulationIn 50 mM HEPES, 100 mM NaCl, 5 µM tetrahydrobiopterin, 25% glycerol, pH 7.4.
      Applications
      Biological Information
      Specific Activity≥25 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 20/21/22-36/37/38

      Harmful by inhalation, in contact with skin and if swallowed.
      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Harmful
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      482721 0

      Documentation

      Nitric Oxide Synthase, Constitutive, Rat Neuronal, Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      482721

      References

      Reference overview
      Abu-Soud, H.M., et al. 1994. J. Biol. Chem. 269, 32047.
      Abu-Soud, H.M., et al. 1994. J. Biol. Chem. 269, 32318.
      Richards, M.K., and Marletta, M.A. 1994. Biochemistry 33, 14723.
      Sheta, E.A., et al. 1994. J. Biol. Chem. 269, 15147.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-September-2008 JSW
      SynonymsnNOS, Rat, Neuronal Nitric Oxide Synthase
      DescriptionRecombinant, rat neuronal NOS expressed in S. frugiperda insect cells using a baculovirus expression system. Useful for studies involving NOS regulation, inhibitor screening and kinetic analysis.
      FormLiquid
      FormulationIn 50 mM HEPES, 100 mM NaCl, 5 µM tetrahydrobiopterin, 25% glycerol, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Combine the following at 37°C: 50 mM        HEPES, pH 7.4 12 µM        BH4 1 mM         CaCl2 20 µg/ml   Calmodulin 100 µM     NADPH 50 µM      Arginine 120 µM     DTT 3 µM        Oxyhemoglobin Measure spectrophotometrically at 401 nm.
      Specific activity≥25 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will produce 1 nmol of nitric oxide per min in the presence of calmodulin at 37°C, pH 7.5.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Harmful
      ReferencesAbu-Soud, H.M., et al. 1994. J. Biol. Chem. 269, 32047.
      Abu-Soud, H.M., et al. 1994. J. Biol. Chem. 269, 32318.
      Richards, M.K., and Marletta, M.A. 1994. Biochemistry 33, 14723.
      Sheta, E.A., et al. 1994. J. Biol. Chem. 269, 15147.

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      Categories

      Life Science Research > Proteins and Enzymes > Other Enzymes