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539576 PKA, Catalytic Subunit, Bovine Heart

PKA, Catalytic Subunit, Bovine Heart MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: cAMP-Dependent Protein Kinase Catalytic Subunit, Protein Kinase A, Catalytic Subunit

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539576
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Overview

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539576-25UG
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      		 Glass bottle 25 μg
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      Description
      OverviewNative PKA catalytic subunit purified from bovine heart. The catalytic subunit of PKA dissociates from the PKA holoenzyme following binding of cAMP to the PKA regulatory subunits and phosphorylates target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser*-X. The purified monomeric catalytic subunit does not require cAMP for activation and is suitable for studies of PKA phosphorylation in vitro with purified substrates or following its introduction into intact cells or cell extracts.
      Note: 25 µg ≥ 500,000 units.
      Catalogue Number539576
      Brand Family Calbiochem®
      SynonymscAMP-Dependent Protein Kinase Catalytic Subunit, Protein Kinase A, Catalytic Subunit
      Application Data
      References
      ReferencesCorbin, J.D., et al. 1988. Methods Enzymol 159, 74.
      Rannels, S.R., et al 1983. Methods Enzymol 99, 55.
      Witt, J.J. and Roskoski, R. Jr. 1975. Anal Biochem. 66, 253.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme required to incorporate 1 pmol phosphate into Kemptide substrate (Cat. No. 05-23-4900) per min at 30°C.
      FormLiquid
      FormulationIn 350 mM K₂HPO₄, 100 µM DTT, pH 6.8.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥20,000 units/µg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      539576-25UG 04055977194739

      Documentation

      PKA, Catalytic Subunit, Bovine Heart SDS

      Title

      Safety Data Sheet (SDS) 

      PKA, Catalytic Subunit, Bovine Heart Certificates of Analysis

      TitleLot Number
      539576

      References

      Reference overview
      Corbin, J.D., et al. 1988. Methods Enzymol 159, 74.
      Rannels, S.R., et al 1983. Methods Enzymol 99, 55.
      Witt, J.J. and Roskoski, R. Jr. 1975. Anal Biochem. 66, 253.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-May-2017 JSW
      SynonymscAMP-Dependent Protein Kinase Catalytic Subunit, Protein Kinase A, Catalytic Subunit
      Application Data
      DescriptionNative PKA catalytic subunit purified from bovine heart. The catalytic subunit of PKA dissociates from the PKA holoenzyme following binding of cAMP to the PKA regulatory subunits and phosphorylates target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser*-X. The purified monomeric catalytic subunit does not require cAMP for activation and is suitable for studies of PKA phosphorylation in vitro with purified substrates or following its introduction into intact cells or cell extracts.
      FormLiquid
      FormulationIn 350 mM K₂HPO₄, 100 µM DTT, pH 6.8.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Kinase Activity Assay: The activity of the PKA catalytic subunit is assayed in 40 mM Tris-HCl, 20 mM magnesium acetate, 200 µM [γ-32P]ATP (500-1000 cpm/pmol) with 130 µM Kemptide substrate in a total volume of 60 µl. The reaction is incubated at 30°C for 5 min and terminated by spotting 40 µl of the reaction mix onto P-81 filters and washing in 0.5% H3PO4 for 5 min for a total of 4 washes. Following the final wash, the filters are rinsed in ethanol, dried, and counted in the presence of scintillation fluid. A value of 200 pmol obtained for Kemptide phosphorylation under these conditions corresponds to 1 pmol phosphorylated casein.
      Purity≥90% by SDS-PAGE
      Specific activity≥20,000 units/µg protein
      Unit definitionOne unit is defined as the amount of enzyme required to incorporate 1 pmol phosphate into Kemptide substrate (Cat. No. 05-23-4900) per min at 30°C.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesCorbin, J.D., et al. 1988. Methods Enzymol 159, 74.
      Rannels, S.R., et al 1983. Methods Enzymol 99, 55.
      Witt, J.J. and Roskoski, R. Jr. 1975. Anal Biochem. 66, 253.