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AP1063 PhosphoDetect™ Anti-PDH-E1α (pSer²³²) Rabbit pAb

This PhosphoDetect™ Anti-PDH-E1α (pSer²³²) Rabbit pAb is validated for use in Immunoblotting, IF, IP for the detection of PDH-E1α (pSer²³²).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Pyruvate Dehydogenase pSer²³² Rabbit pAb

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AP1063
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

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AP1063-50UG
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      Description
      Overview

      This product has been discontinued.



      Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser232 in HEK293 cells.
      Catalogue NumberAP1063
      Brand Family Calbiochem®
      SynonymsAnti-Pyruvate Dehydogenase pSer²³² Rabbit pAb
      Application Data
      Detection of human PDH-E1α, phosphorylated at Ser293, Ser300, and Ser232 by immunoblotting. Samples: Whole tissue extract from mouse liver (20 µg) left untreated (lane 1) or treated with dichloroacetate (DCA, 5 mM for 4 h) (lane 2). Primary antibody: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) PhosphoDetect™ Anti PDH-E1α (pSer300) Rabbit pAb (Cat. No. AP1064) PhosphoDetect™ Anti PDH-E1α (pSer232) Rabbit pAb (Cat. No. AP1063) all used at 0.25 µg/ml and Anti-PDHE-1α. Detection: chemiluminescence. Data courtesy of Matthew Rardin, University of California San Diego.

      Detection of PDH-E1α, phosphorylated at Ser293, Ser300, and Ser232 by immunocytochemistry. Samples: COS7 cells incubated in presence dichloroacetate (DCA; 5 mM for 2 h). All samples were incubated with MitoTracker® Red stain (100 nM for 20 min; to visualize mitochondria) and fixed in 3.7% formaldehyde, followed by methanol (3 min at -20°C), and permeabilization in PBS, 6% BSA, 0.3% Triton™ X-100 detergent, 0.1% Tween® 20 detergent. Primary antibodies: PhosphoDetect™ Anti-PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (panel B), PhosphoDetect™ Anti-PDH-E1α (pSer300) Rabbit pAb (Cat. No. AP1064) (panel C), PhosphoDetect™ Anti-PDH-E1α (pSer232) Rabbit pAb (Cat. No. AP1063) (panel D) (0.5 µg/ml, green fluorescence) and anti-PDH-E1α antibody (green fluorescence, top 3 panels) (panel A). Detection: fluorescence (Alexa Fluor® 488 secondary antibody) with DAPI counterstain (300 nM, 1 min, blue fluorescence). Data courtesy of Matthew Rardin, University of California San Diego.
      References
      ReferencesRardin M.J., et. al. 2009. Anal. Biochem. 2, 157.
      Seifert, F., et al. 2007. Biochemistry 21, 6277.
      Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
      Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans. 34, 217.
      Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlHEK293 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ200
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesImmunoblotting (0.25 µg/ml)
      Immunofluorescence (Please see comments)
      Immunoprecipitation (1 µg/ml)
      Application CommentsThis antibody has been known to work with immunofluorescence. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³² phosphorylation site of human PDH-E1α
      ImmunogenHuman
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1063-50UG 04055977227789

      Documentation

      PhosphoDetect™ Anti-PDH-E1α (pSer²³²) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-PDH-E1α (pSer²³²) Rabbit pAb Certificates of Analysis

      TitleLot Number
      AP1063

      References

      Reference overview
      Rardin M.J., et. al. 2009. Anal. Biochem. 2, 157.
      Seifert, F., et al. 2007. Biochemistry 21, 6277.
      Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
      Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans. 34, 217.
      Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-December-2010 RFH
      SynonymsAnti-Pyruvate Dehydogenase pSer²³² Rabbit pAb
      ApplicationImmunoblotting (0.25 µg/ml)
      Immunofluorescence (Please see comments)
      Immunoprecipitation (1 µg/ml)
      Application Data
      Detection of human PDH-E1α, phosphorylated at Ser293, Ser300, and Ser232 by immunoblotting. Samples: Whole tissue extract from mouse liver (20 µg) left untreated (lane 1) or treated with dichloroacetate (DCA, 5 mM for 4 h) (lane 2). Primary antibody: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) PhosphoDetect™ Anti PDH-E1α (pSer300) Rabbit pAb (Cat. No. AP1064) PhosphoDetect™ Anti PDH-E1α (pSer232) Rabbit pAb (Cat. No. AP1063) all used at 0.25 µg/ml and Anti-PDHE-1α. Detection: chemiluminescence. Data courtesy of Matthew Rardin, University of California San Diego.

      Detection of PDH-E1α, phosphorylated at Ser293, Ser300, and Ser232 by immunocytochemistry. Samples: COS7 cells incubated in presence dichloroacetate (DCA; 5 mM for 2 h). All samples were incubated with MitoTracker® Red stain (100 nM for 20 min; to visualize mitochondria) and fixed in 3.7% formaldehyde, followed by methanol (3 min at -20°C), and permeabilization in PBS, 6% BSA, 0.3% Triton™ X-100 detergent, 0.1% Tween® 20 detergent. Primary antibodies: PhosphoDetect™ Anti-PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (panel B), PhosphoDetect™ Anti-PDH-E1α (pSer300) Rabbit pAb (Cat. No. AP1064) (panel C), PhosphoDetect™ Anti-PDH-E1α (pSer232) Rabbit pAb (Cat. No. AP1063) (panel D) (0.5 µg/ml, green fluorescence) and anti-PDH-E1α antibody (green fluorescence, top 3 panels) (panel A). Detection: fluorescence (Alexa Fluor® 488 secondary antibody) with DAPI counterstain (300 nM, 1 min, blue fluorescence). Data courtesy of Matthew Rardin, University of California San Diego.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser232.
      BackgroundThe pyruvate dehydogenase (PDH) complex (PDC) catalyzes the irreversible conversion of pyruvate to acetyl CoA, providing the link between glycolyis and the TCA cycle. Dysregulation of the PDC in skeletal muscle has been implicated in type 2 diabetes and mutations in the PDC result in various mitochondrial diseases. The PDC is a multienzyme complex composed of several copies of three catalytic proteins (E1, E2, and E3): one structural (E3-binding protein) and two regulatory proteins (PDH kinase [PDK] and PDH phosphatase [PDP]). The activity of the PDC is mainly regulated by phosphorylation-dephosphorylation of three specific serine residues (232, 293, 300) on the E1 subunit. Phosphorylation of PDH determines the activity of the complex. Phosphorylation by one of four PDK isoforms inactivates the complex, while removal of the phosphate by one of two PDP isoforms activates the complex. The activity of the PDP1 isoform is sensitive to Ca2+, while the PDK isoforms are regulated by the reduction and acetylation status of the E2 subunit, pyruvate, and the small molecule the anti cancer dichloroacetate. (DCA).
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³² phosphorylation site of human PDH-E1α
      Specieshuman, mouse, rat
      Positive controlHEK293 cells
      FormLiquid
      FormulationIn PBS.
      Preservative≤0.1% sodium azide
      CommentsThis antibody has been known to work with immunofluorescence. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesRardin M.J., et. al. 2009. Anal. Biochem. 2, 157.
      Seifert, F., et al. 2007. Biochemistry 21, 6277.
      Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
      Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans. 34, 217.
      Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.