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PF073 TGF-β3, Human, Recombinant, S. frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Transforming Growth Factor-β3

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PF073
  
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      Overview

      Replacement Information

      Key Spec Table

      Purity
      ≥95% by SDS-PAGE
      Description
      Overview

      This product has been discontinued.



      Recombinant, human transforming growth factor-β3 expressed in S. frugiperda insect cells.
      Catalogue NumberPF073
      Brand Family Calbiochem®
      SynonymsTransforming Growth Factor-β3
      References
      ReferencesMehler, M.F. and Kessler, J.A. 1995. Crit. Rev. Neurobiol. 9, 419.
      Burton, P.B.J., et al. 1993. Int. J. Expl. Pathol. 74, 87.
      Massague, J., et al. 1992. Cell 69, 1067.
      Sporn, M.B. and Roberts, A.B. 1992. J. Cell Biol. 119, 1017.
      Kondaiah, P., et al. 1990. J. Biol. Chem. 265, 1089.
      Massague, J. 1990. Ann. Rev. Cell Biol. 6, 597.
      Roberts, A.B. and Sporn, M.B. 1990. In Peptide Growth Factors and Their Receptors, ed. M. Sporn, A.B. Roberts. Heidelberg: Springer-Verlag.
      Tsang, M., et al. 1990. Lymphokine Res. 9, 607.
      Lyons, R.M., et al. 1988. J. Cell Biol. 106, 1659.
      Madisen, L., et al. 1988. DNA 7, 1.
      Ten Dijke, P., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 4715.
      Derynck, R., et al. 1987. Nucleic Acids Res. 15, 3188.
      Massague, J. 1987. Cell 49, 437.
      Product Information
      FormLyophilized
      FormulationLyophilized from 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg of cytokine.
      PreservativeNone
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityEC₅₀ = 0.01-0.03 ng/ml as determined by the ability to inhibit IL-4-dependent ³H-thymidine incorporation in mouse HT-2 cells.
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤1.0 EU/µg cytokine
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Purified human recombinant TGF-β3 is an extremely hydrophobic protein that adheres strongly to surfaces.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PF073 0

      Documentation

      TGF-β3, Human, Recombinant, S. frugiperda SDS

      Title

      Safety Data Sheet (SDS) 

      TGF-β3, Human, Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      PF073

      References

      Reference overview
      Mehler, M.F. and Kessler, J.A. 1995. Crit. Rev. Neurobiol. 9, 419.
      Burton, P.B.J., et al. 1993. Int. J. Expl. Pathol. 74, 87.
      Massague, J., et al. 1992. Cell 69, 1067.
      Sporn, M.B. and Roberts, A.B. 1992. J. Cell Biol. 119, 1017.
      Kondaiah, P., et al. 1990. J. Biol. Chem. 265, 1089.
      Massague, J. 1990. Ann. Rev. Cell Biol. 6, 597.
      Roberts, A.B. and Sporn, M.B. 1990. In Peptide Growth Factors and Their Receptors, ed. M. Sporn, A.B. Roberts. Heidelberg: Springer-Verlag.
      Tsang, M., et al. 1990. Lymphokine Res. 9, 607.
      Lyons, R.M., et al. 1988. J. Cell Biol. 106, 1659.
      Madisen, L., et al. 1988. DNA 7, 1.
      Ten Dijke, P., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 4715.
      Derynck, R., et al. 1987. Nucleic Acids Res. 15, 3188.
      Massague, J. 1987. Cell 49, 437.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-September-2008 RFH
      SynonymsTransforming Growth Factor-β3
      DescriptionRecombinant, human transforming growth factor-β3 expressed in S. frugiperda insect cells. The disulfide-linked homodimeric human recombinant TGF-β3 contains two 112 amino acid peptides and has a predicted molecular weight of ~25 kDa. Useful for growth ihibition assays and other assays designed to study cellular responses and receptor interactions involving TGF. The transforming growth factor (TGF) superfamily contains structurally related secretory peptides, which become activated by proteolytic cleavage. In addition to the TGF-βs, other family members include the activins, inhibins, bone morphogenetic proteins (BMPs), glial cell-line derived neurotrophic factor (GDNF), and Mullerian inhibitory substance (MIS). The three TGF-β isoforms, TGF-β1, TGF-β2, and TGF-β3, are potent regulators of cell growth and differentiation, extracellular matrix formation, and inflammatory events. The TGF-βs bind to three high affinity serine/threonine kinase receptors, designated type I (~55-65 kDa), type II (~70-85 kDa), and type III/betaglycan (~200-400 kDa). Structurally, the TGF-β1, β2, and β3 proteins are homodimers composed of disulfide linked chains 112 amino acids in length. Composition of TGF-β proteins from different species is highly conserved at the DNA level as well as at the protein level showing in general >85% identity within a subtype and 64-82% identity between subtypes. TGF-β1 and β2 were first identified on the basis of their biological activity and subsequently cloned from expression libraries. Human TGF-β3 was identified first at the cDNA level and of the three major isoforms, appears to be the most biologically active. The expression of TGF-β3 at various stages of morphogenesis and at different sites suggests an important role in the developmental process.
      FormLyophilized
      FormulationLyophilized from 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg of cytokine.
      Purity≥95% by SDS-PAGE
      ContaminantsEndotoxin: ≤1.0 EU/µg cytokine
      Biological activityEC₅₀ = 0.01-0.03 ng/ml as determined by the ability to inhibit IL-4-dependent ³H-thymidine incorporation in mouse HT-2 cells.
      SolubilityReconstitute in sterile 4 mM HCl containing at least 0.1% human serum albumin or bovine serum albumin to a final concentration of ≥1 µg/ml.
      PreservativeNone
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Purified human recombinant TGF-β3 is an extremely hydrophobic protein that adheres strongly to surfaces.
      Toxicity Standard Handling
      ReferencesMehler, M.F. and Kessler, J.A. 1995. Crit. Rev. Neurobiol. 9, 419.
      Burton, P.B.J., et al. 1993. Int. J. Expl. Pathol. 74, 87.
      Massague, J., et al. 1992. Cell 69, 1067.
      Sporn, M.B. and Roberts, A.B. 1992. J. Cell Biol. 119, 1017.
      Kondaiah, P., et al. 1990. J. Biol. Chem. 265, 1089.
      Massague, J. 1990. Ann. Rev. Cell Biol. 6, 597.
      Roberts, A.B. and Sporn, M.B. 1990. In Peptide Growth Factors and Their Receptors, ed. M. Sporn, A.B. Roberts. Heidelberg: Springer-Verlag.
      Tsang, M., et al. 1990. Lymphokine Res. 9, 607.
      Lyons, R.M., et al. 1988. J. Cell Biol. 106, 1659.
      Madisen, L., et al. 1988. DNA 7, 1.
      Ten Dijke, P., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 4715.
      Derynck, R., et al. 1987. Nucleic Acids Res. 15, 3188.
      Massague, J. 1987. Cell 49, 437.

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      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors