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WB59 Western-Re-Probe™ Reagent

This Western-Re-Probe™ Reagent is validated for use in Immunoblotting.

Western-Re-Probe™ Reagent MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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WB59
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Overview

Replacement Information

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WB59-100ML
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      		 Plastic ampoule 100 ml
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      Description
      OverviewDesigned to dissociate and remove antibodies from membrane-bound proteins without destroying antigenic binding capacity. Recommended for stripping chemiluminescent or radioisotopic signals from blots. Not recommended for stripping color-producing Western blots. Supplied as a 5X solution sufficient for stripping 25-30 standard blots.
      A special formulation designed to dissociate and remove antibodies from the membrane bound protein without destroying the antigenic binding affinity.
      Catalogue NumberWB59
      Brand Family Calbiochem®
      References
      Product Information
      FormLiquid
      FormulationSupplied as a 5X solution.
      Kit contains5X Re-Probe solution (125 ml)
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (see comments)
      Application CommentsSufficient volume to strip 25-30 standard blots.

      ADVANTAGES
      -Immunoblotting analyzed repeatedly with multiple probes
      -Saving on hard-to-get reagents and samples
      -Correction and re-analysis of unsatisfactory Western Blots
      -Saving time spent in gel re-running and transfer processes

      PROTOCOL
      1- Keep the membrane wet in an appropriate buffer after each probing procedure. Prepare 20-25 ml solution of 1X Western-Re-Probe™ in water.
      2- Incubate the membrane in 1X Western-Re-Probe™ buffer for 30 min at room temperature (RT) with shaking.
      3- Wash the membrane three times in PBST or TBST buffer containing 0.1% Tween®-20 detergent. Use a large volume of washing buffer in a tray for the washing step. Each wash cycle involves agitating the membrane 5 min in the washing buffer.
      4- Block the membrane and perform immunodetection.

      Western-Re-Probe™ Reagent can be used for stripping and re-probing membranes 2-3 times. Some loss of signal may be noticed after second cycle of stripping and re-probing.

      PROTOCOL SUMMARY
      1. Incubate membrane in 1X Western-Re-Probe™ (30 min at RT) with shaking
      2. Wash the membrane 3 times in PBST or TBST buffer
      3. Block membrane and perform immunodetection
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Kit contains5X Re-Probe solution (125 ml)
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      WB59-100ML 04055977206746

      Documentation

      Western-Re-Probe™ Reagent SDS

      Title

      Safety Data Sheet (SDS) 

      Western-Re-Probe™ Reagent Certificates of Analysis

      TitleLot Number
      WB59
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-July-2009 RFH
      ApplicationImmunoblotting (see comments)
      DescriptionWestern-Re-Probe™ Reagent is specifically formulated to dissociate and remove antibodies from the membrane bound protein without destroying the antigenic binding affinity. The removal of antibodies also removes corresponding chemiluminescence or radioactive signals from the blots. Stripped blots can be probed with new probes.
      FormLiquid
      FormulationSupplied as a 5X solution.
      CommentsSufficient volume to strip 25-30 standard blots.

      ADVANTAGES
      -Immunoblotting analyzed repeatedly with multiple probes
      -Saving on hard-to-get reagents and samples
      -Correction and re-analysis of unsatisfactory Western Blots
      -Saving time spent in gel re-running and transfer processes

      PROTOCOL
      1- Keep the membrane wet in an appropriate buffer after each probing procedure. Prepare 20-25 ml solution of 1X Western-Re-Probe™ in water.
      2- Incubate the membrane in 1X Western-Re-Probe™ buffer for 30 min at room temperature (RT) with shaking.
      3- Wash the membrane three times in PBST or TBST buffer containing 0.1% Tween®-20 detergent. Use a large volume of washing buffer in a tray for the washing step. Each wash cycle involves agitating the membrane 5 min in the washing buffer.
      4- Block the membrane and perform immunodetection.

      Western-Re-Probe™ Reagent can be used for stripping and re-probing membranes 2-3 times. Some loss of signal may be noticed after second cycle of stripping and re-probing.

      PROTOCOL SUMMARY
      1. Incubate membrane in 1X Western-Re-Probe™ (30 min at RT) with shaking
      2. Wash the membrane 3 times in PBST or TBST buffer
      3. Block membrane and perform immunodetection
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Toxicity Standard Handling