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SCC245 YUMMER1.7-H2B-GFP5 Mouse Melanoma Cell Line

The YUMMER1.7-H2B-GFP5 mouse melanoma cell line is a valuable model for studies of immune checkpoint inhibition and mechanisms of anti-tumor responses.

SCC245
≥1X10⁶ cells/vial   
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC245
      DescriptionYUMMER1.7-H2B-GFP5 Mouse Melanoma Cell Line
      OverviewThe YUMMER1.7 H2B-GFP mouse melanoma cell line is both immunocompetent and reflective of the somatic mutations common in melanomas. The YUMMER1.7 H2B-GFP cell line carries three driver mutations of melanoma: Braf V600E, Pten -/- and Cdkn2 -/- (1,2). In addition, the YUMMER1.7 H2B-GFP cell line harbors a high frequency of stable UV-induced somatic mutations which have been shown to stimulate host adaptive immune response (3). YUMMER1.7-H2B-GFP cells stably express GFP fused to histone H2B, allowing visualization of chromosomal dynamics and facilitating in vivo detection of YUMMER1.7 melanoma cells and YUMMER1.7-derived tumors. The unique features of the YUMMER1.7 H2B-GFP cell line make it a valuable model for studies of immune checkpoint inhibition and mechanisms of anti-tumor responses.

      Source:
      The YUMMER1.7 H2B-GFP mouse melanoma cell line is a tetraploid clonal isolate derived from YUMM1.7 cells exposed to UVB radiation into which a histone H2B-GFP lentiviral fusion construct has been stably introduced via transduction (4). The original YUMM1.7 cell line was derived from a 4-hydroxytamoxifen-induced melanoma tumor in a male C57/B1/6 mouse into which mutations from the Braf/Pten genetically-engineered mouse model had been introduced via backcrossing (1). The YUMMER1.7 H2B-GFP cell line harbors the Braf V600E mutation and is homozygous negative for wild-type Pten and Cdkn2 (3).
      Alternate Names
      • Yale University Mouse Melanoma Exposed to Radiation 1.7
      Background InformationThe great promise of immune-based therapies in cancer and recent progress in successful application of these approaches has brought to the fore the necessity of immune-competent models to evaluate immune system responses to cancer cells. Melanomas exhibit relatively high somatic mutation burden, and these mutations can act as neoantigens that generate anti-tumor immune responses. The development of immunocompetent cell models is critical to the advancement of cancer immunotherapy and understanding of immune responses, although few tractable model systems are available.

      References:
      1. Meeth K et al. (2016) The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597.
      2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten loss to induce metastatic melanoma. Nat Genet. 41(5): 544-552.
      3. Wang J et al. (2017) UV-induced somatic mutations elicit a functional T cell response in the YUMMER1.7 mouse melanoma model. Pigment Cell Melanoma Res 30(4): 428-435.
      4. Zhou T et al. (2020) IL-18BP is a secreted immune checkpoint and barrier to IL-18 immunotherapy Nature 583(7817):609-614.
      References
      Product Information
      Applications
      ApplicationThe YUMMER1.7-H2B-GFP5 mouse melanoma cell line is a valuable model for studies of immune checkpoint inhibition and mechanisms of anti-tumor responses.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
      • Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC245 04061841074977

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