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1.50447

SeQuant® ZIC®-HILIC 200Å (3.5 µm) HPLC Columns

L × I.D. 100 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

别名:

两性亲水作用液相色谱HPLC柱

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关于此项目

UNSPSC Code:
41115709
NACRES:
NB.21
Particle size:
3.5 μm
Matrix active group:
ZIC-HILIC, ZIC-HILIC bonding phase
Pore size:
200 Å pore size
Matrix:
fully porous particle
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产品名称

SeQuant® ZIC-HILIC™ 3.5µm,200Å 100 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC

material

PEEK-lined stainless steel column

Quality Level

100

agency

suitable for USP L114

product line

SeQuant®

feature

endcapped: no

parameter

550 bar pressure, 70 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)

column L × I.D.

10 cm × 2.1 mm

matrix

fully porous particle

matrix active group

ZIC-HILIC, ZIC-HILIC bonding phase

particle size

3.5 μm

pore size

200 Å pore size

operating pH

2-7.5

separation technique

hydrophilic interaction (HILIC)

storage temp.

no temp limit

General description

SeQuant® ZIC®-HILIC亲水作用色谱柱专用于保留和分离所有类型的极性和亲水性化合物，实现高选择性和重现性的可靠色谱分析。 ZIC®-HILIC填料的硅胶基质键合着强极性永久两性离子。两性离子电荷1:1平衡，使ZIC®-HILIC色谱柱整体呈中性，具有微弱但重要的离子作用，更利于发挥分离选择性。进行ZIC®-HILIC色谱柱的方法开发时，不受pH影响的永久两性离子更方便选择性调整，保证在优化洗脱剂时，只有分析物受到影响（色谱柱不受影响）。色谱柱的200 Å孔径使其成为在亲水作用色谱条件下分离极性大分子的理想选择。250 mm x 2.1 mm内径的色谱柱规格驻留体积小，是使用UHPLC或优化的HPLC仪器分离复杂样品的最佳选择。优越的保留能力和稳健可靠的性能使得SeQuant® HILIC色谱柱在全球无数实验室备受欢迎。比如，美国FDA（美国食药监）已经推荐SeQuant® ZIC®-HILIC色谱柱用于三聚氰胺及相关物质的分析。SeQuant® HILIC色谱柱具有高稳定和低流失的优点，因而十分适合对背景要求较高的LC-MS应用，高背景会导致信号抑制，并干扰定量检测，同时还会更加损害仪器。高稳定性也令这种高效HILIC色谱柱同样适合传统的HPLC分析。详见Supelco HPLC色谱柱系列和方法开发资料：手册：HPLC和UHPLC色谱柱选择指南 手册：HPLC实用指南——方法开发指南

Analysis Note

理论塔板数（N/m）（胞嘧啶）： ≥ 90000
对称性（胞嘧啶）：0.95 - 1.40

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany

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Selectivity Examination of Stationary Phases for HILIC

This article evaluates different phase chemistries for stationary phases in Hydrophilic Interaction Liquid Chromatography (HILIC) and uses multivariate analysis for classification based on chemical modification.

Liquid chromatography-mass spectrometry metabolite library for metabolomics: Evaluating column suitability using a scoring approach
Diamantidou D, et al.
Journal of Chromatography A, 1690, 463779-463779 (2023)
Dimitra Diamantidou et al.
Journal of chromatography. A, 1690, 463779-463779 (2023-01-22)
Untargeted metabolomic studies require an extensive set of analyte (metabolic) information to be obtained from each analyzed sample. Thus, highly selective, and efficient analytical methodologies together with reversed-phase (RP) or hydrophilic interaction liquid chromatography (HILIC) are usually applied in these

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货号GTIN
1.50447.000104027269134297