R6513
核糖核酸酶A 来源于牛胰腺
Molecular Biology, ≥70 Kunitz units/mg protein, lyophilized
别名:
RNAsea, RNase A, 核糖核酸 3′-嘧啶寡核苷酸水解酶, 核糖核酸酶 I, 胰核糖核酸酶
选择尺寸
关于此项目
grade
Molecular Biology
Quality Level
form
lyophilized
specific activity
≥70 Kunitz units/mg protein
mol wt
13.7 kDa, ~13,700
foreign activity
Exonuclease and endonuclease, none detected, NICKase and DNase, none detected
storage temp.
−20°C
SMILES string
[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
General description
Application
- RNase A用于去除DNA质粒和基因组DNA制品和蛋白质样品中的RNA。
- RNase A还用于RNA序列分析和保护测定。
- RNase A已用作计算辅助药物设计的工具。
- RNase A为RNA序列分析提供支持。
- RNase A水解蛋白质样品中的RNA。
- RNase A为DNA纯化提供支持。
- 质粒DNA纯化
- 在细胞核染色过程中，酶解双链RNA并避免染色
- 免疫过氧化物酶和免疫荧光检测
- 流式细胞分析前的细胞处理
- 用作杂交后缓冲液组分，在原位杂交后洗涤FFPE组织切片
- 在制备外膜蛋白(OMP)提取物期间处理细胞
- 核糖核酸酶保护试验
- 去除非特异性结合的RNA
- 核糖核酸序列的分析
- 水解蛋白质样品所含的RNA
Features and Benefits
Analysis Note
Other Notes
该RNase A产品不需要煮沸原液以灭活剩余的DNase，这有可能导致RNase沉淀，并可能导致酶活性损失。如果RNase溶液在中性pH下加热，就会发生沉淀。当在较低的pH值下加热时，由于存在蛋白质杂质，可能会发生一些沉淀。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
实验方案
本实验方案可用于测定核糖核酸酶A(RNase A)的活性。
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
商品
Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein
Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein
Separation of null
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