05-777
Anti-Phosphotyrosine Antibody, recombinant clone 4G10®
clone 4G10®, Upstate®, from mouse
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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
4G10®, monoclonal
Species reactivity:
vertebrates
Application:
IP, WB
Citations:
27
Servicio técnico
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Permítanos ayudarlebiological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4G10®, monoclonal
species reactivity
vertebrates
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2bκ
shipped in
wet ice
target post-translational modification
phosphorylation (pTyr)
General description
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
varies
Immunogen
Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via Nickel affinity matrices.
Application
Anti-Phosphotyrosine Antibody, clone 4G10 is a recombinant antibody that detects tyrosine phosphorylated proteins in all species. This unique recombinant antibody is validated for use in IC, IH, IP, WB and is published in peer review journals.
Physical form
Format: Purified
Protein G purified recombinant 4G10 mouse IgG2bk in 0.01M Phosphate Buffer containing no preservative, pH 7.1
Analysis Note
Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells
Legal Information
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Cytosolic lysine residues enhance anterograde transport and activation of the erythropoietin receptor.
Liron Yosha,Orly Ravid,Nathalie Ben-Califa,Drorit Neumann
The Biochemical Journal null
Kazunobu Tachibana et al.
Molecular and cellular biology, 25(11), 4693-4702 (2005-05-19)
The development of the cardiovascular system and the development of the early hematopoietic systems are closely related, and both require signaling through the Tie2 receptor tyrosine kinase. Although endothelial cells and hematopoietic cells as well as their precursors share common
Trond Methi et al.
European journal of immunology, 38(9), 2557-2563 (2008-09-16)
T cells with short interfering RNA-mediated Lck-knockdown (kd) display paradoxical hyper-responsiveness upon TCR ligation. We have previously reported a possible mechanism for T-cell activation in cells with low levels of Lck depending on Grb2-SOS1 recruitment to the zeta-chain of TCR/CD3
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| 05-777 | 04053252371110 |