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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
mouse, human, rat
Application:
IP, WB
Citations:
23
Servicio técnico
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Permítanos ayudarleNombre del producto
Anti-MAP Kinase 2/Erk2 Antibody, Upstate®, from rabbit
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human, rat
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... MAPKAPK2(9261)
mouse ... Mapkapk2(17164)
rat ... Mapkapk2(289014)
General description
42kDa
Immunogen
Recombinant mouse ERK2
Application
Anti-MAP Kinase 2/Erk2 Antibody detects level of MAP Kinase 2/Erk2 & has been published & validated for use in IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
Biochem/physiol Actions
MAP Kinase encoded by the mapk gene
Physical form
0.02M phosphate buffer, pH 7.6, 0.25M NaCl, 0.1% sodium azide
Format: Purified
Protein G Chromatography
Preparation Note
2 years at -20°C
Analysis Note
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on mouse 3T3/A31 fibroblasts, human A431 carcinoma cells or rat L6 skeletal fibroblasts
Other Notes
Replaces: 04-349
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Clase de almacenamiento
12 - Non Combustible Liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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S A Barber et al.
Journal of immunology (Baltimore, Md. : 1950), 155(3), 1404-1410 (1995-08-01)
LPS-stimulated macrophages (M phi) produce inflammatory mediators that are largely responsible for the pathophysiology associated with septic shock. M phi respond to LPS with rapid protein phosphorylation and dephosphorylation on serine, threonine, and tyrosine residues. If these events are critical
Suppression of c-Myc-induced apoptosis by Ras signalling through PI(3)K and PKB
Kauffmann-Zeh, A., et al
Nature, 385, 544-548 (1997)
Dual phosphorylation and autophosphorylation in mitogen-activated protein (MAP) kinase activation.
Her, J H, et al.
The Biochemical Journal, 296 ( Pt 1), 25-31 (1993)
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| 06-333 | 04053252280252 |