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Merck

234154

Human Collagen Type IV

from human placenta, liquid, suitable for cell culture

Sinónimos:

Collagen, Type IV, Human Placenta, Native, type IV collagen

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Número CAS:
9007-34-5
MDL number:
MFCD00130825
UNSPSC Code:
12352202
NACRES:
NA.77

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Nombre del producto

Collagen, Type IV, Human Placenta, (100 μg), solution, suitable for cell culture, purified by (Highly purified), Native protein

biological source

human placenta

description

Merck USA index - 14, 2480

assay

≥90% (SDS-PAGE)

form

solution

purified by

(Highly purified)

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

concentration

(100 μg)

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

Application

The human placental collagen type IV (Sigma Aldrich 234154) is used:

  • as a coating substrate for cell culture treated flasks to promote the growth and adhesion of CuFi-1 cells, providing reliable conditions for cell line propagation and maintenance
  • to coat transwell inserts, providing a suitable substrate for the growth and formation of monolayers in intestinal organoid culture for trans epithelial electrical resistance (TEER) measurement and drug treatments
  • to coat inserts for two-dimensional monolayer culture

Biochem/physiol Actions

Collagen Type IV interacts with numerous binding partners. Type IV collagen forms a structural scaffold within the basement membrane (BM) along with other macromolecules like laminins, heparan sulfate proteoglycans, fibronectin, and entactin. This complex network regulates the interaction between the BM and adhering cells, providing critical support and functionality. Thereby, regulates cell adhesion and migration.

Disclaimer

Toxicity: Standard Handling (A)

General description

Collagen Type IV is a primary component of the basement membrane (BM). It is a specialized sheet-like extracellular matrix that surrounds specific cell types such as skeletal muscle cells, smooth muscle cells, heart muscle cells, and adipocytes in multicellular tissues. Collagen type IV is characterized as a nonfibril-forming collagen and is composed of six genetically distinct α-chain polypeptides. These chains have the potential to form 56 different combinations, resulting in various homotrimers. Through self-assembly, collagen type IV constructs organized networks that play a crucial role in maintaining the stability and assembly of the basement membrane (BM). Its presence is essential for the structural integrity and functionality of the BM.

Other Notes

Miller, E.J., and Gay, S. 1987. Methods Enzymol. 144, 3.
Klasson, S.C., et al. 1986. Coll. Relat. Res.6, 397.
Glanville, R.W., et al. 1979. Eur. J. Biochem.95, 383.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In sterile 2 mM HCl.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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