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362185

PNGase F Glycosidase

Name: PNGase F Glycosidase
Brand: MM

Removes all N-linked glycans, suitable for mass spectrometry, from Elizabethkingia meningosepticum

Sinónimos:

N-Glycosidase F, Elizabethkingia meningosepticum, Glycopeptidase F, PNGase F

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About This Item

Número CAS:

83534-39-8

Número CE:

3.5.1.52 (BRENDA, IUBMB)

Número MDL:

MFCD00131222

Código UNSPSC:

12352202

NACRES:

NA.54

Nombre del producto

N-Glycosidase F, Elizabethkingia meningosepticum,

origen biológico

bacterial (Elizabethkingia meningosepticum)

Nivel de calidad

100

conjugado

(N-linked)

Formulario

liquid

actividad específica

≥20,000 units/mg protein
≥4500 units/mL

fabricante / nombre comercial

Calbiochem^®

condiciones de almacenamiento

do not freeze

actividad extraña

Proteases, none detected

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

Descripción general

Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1,3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.

Forma física

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

Otras notas

Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.

One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 nmol denatured ribonuclease B per min at 37°C, pH 7.5. Cleavage is monitored by SDS-PAGE. One unit of N-Glycosidase F is equal to 1 IUB milliunit.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Toxicity: Standard Handling (A)

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)