393206
Anti-HNE Fluorophore Rabbit pAb
liquid, Calbiochem®
Sinónimos:
Anti-N α-Acetyllysine-4-hydroxy-2-nonenal Fluorophore, Anti-Nα-Acetyllysine-4-hydroxy-2-nonenal Fluorophore
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
Acerca de este artículo
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
-
Application:
—
Citations:
16
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarlebiological source
rabbit
Quality Level
antibody form
diluted serum
antibody product type
primary antibodies
clone
polyclonal
form
liquid
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA (1:500-1:1000)
Immunoblotting (1:1000-1:5000)
Immunohistochemistry (1:100-1:500))
isotype
IgG
shipped in
wet ice
storage temp.
−70°C
target post-translational modification
unmodified
General description
Anti-HNE Fluorophore, rabbit polyclonal, recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore. It is validated for use in ELISA, Western blotting, and immunohistochemistry.
Rabbit polyclonal antibody supplied as diluted serum. Recognizes the HNE-derived chromophore of the 2:1 lysine-HNE fluorophore.
Recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore.
Immunogen
a 2:1 Nα-acetyllysine-HNE fluorophore, conjugated to KLH
Application
ELISA (1:500-1:1000)
Immunoblotting (1:1000-1:5000)
Immunohistochemistry (1:100-1:500)
Immunoblotting (1:1000-1:5000)
Immunohistochemistry (1:100-1:500)
Physical form
Serum diluted 1:1 with glycerol.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Due to the small epitope size, overnight incubation with the antibody greatly increases sensitivity by immunoblotting. Determined by competitive ELISA and immunoblotting to recognize the chromophore structure of fluorophore derived from the reaction of 4-HNE with primary amines. Does not recognize 1:1 cysteine-, 1:1 histidine-, or 1:1 lysine-HNE Michael Adducts. Variables associated with assay conditions will dictate the proper working dilution.
Tsai, L., et al. 1998. Proc. Natl. Acad. Sci. USA95, 7975.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Still not finding the right product?
Pruebe nuestra Herramienta de selección de productos para limitar sus opciones
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Ernst-Bernhard Kayser et al.
PloS one, 11(1), e0148219-e0148219 (2016-01-30)
Lack of NDUFS4, a subunit of mitochondrial complex I (NADH:ubiquinone oxidoreductase), causes Leigh syndrome (LS), a progressive encephalomyopathy. Knocking out Ndufs4, either systemically or in brain only, elicits LS in mice. In patients as well as in KO mice distinct
Erik P Rader et al.
Rejuvenation research, 20(2), 93-102 (2016-07-06)
Exercise is the most accessible, efficacious, and multifactorial intervention to improve health and treat chronic disease. High-intensity resistance exercise, in particular, also maximizes skeletal muscle size and strength-outcomes crucial at advanced age. However, such training is capable of inducing muscle
Mara Laslo et al.
Age (Dordrecht, Netherlands), 35(4), 1117-1132 (2012-05-29)
Superoxide dismutase 1 (SOD1), a critical enzyme against oxidative stress, is implicated in aging and degenerative diseases. We previously showed that a nutraceutical containing freeze-dried açai pulp promotes survival of flies fed a high-fat diet or sod1 knockdown flies fed