Anti-mouse BID cleavage (59/60) site specific Antibody

BH3 interacting domain death agonist (BID, 22 kDa) is a pro-apoptotic member of the Bcl-2 family. BID interacts with both Bcl-2 and Bax through its BH3 domain. It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase-8 in response to TNF-α or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase-8 activation and mitochondrial death machinery.

The antiserum was produced against a chemically synthesized peptide derived from the 15 kDa caspase-8 cleavage product of mouse BID.

Research Category
Apoptosis & Cancer

Research Sub Category
BCL2 & Inhibition

This Anti-mouse BID cleavage (59/60) site specific Antibody is validated for use in WB for the detection of mouse BID cleavage (59/60) site specific.

Mouse 15 kDa caspase-8 cleavage product of BID. Human (75% conserved) BID has been tested and shown not to cross-react. Rat (100% conserved) and chicken (62% conserved) BID have not been tested.

Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier, 0.05% sodium azide.

Format: Purified

Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.

Control
Mouse L929 (3T3-L1) cells treated with TNF-α to induce cleavage; caspase-8 cleaved recombinant mouse BID.

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