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Merck

AB1548

Anti-NMDAR2A&B Antibody, pan

Chemicon®, from rabbit

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IHC (p), IP, WB
Citations:
44

Documentos clave

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, guinea pig, rat, human, rabbit, avian

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable

NCBI accession no.

NM_000833.2

UniProt accession no.

Q12879, Q13224

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... GRIN2A(2903)

Immunogen

Synthetic peptide (LNSCSNRRVYKKMPSIESDV) corresponding to the C-terminus of rat NMDAR2A receptor subunit conjugated to BSA.

Application

Anti-NMDAR2A&B Antibody, pan detects level of NMDAR2A&B & has been published & validated for use in IH(P), IC, IH, IP & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Western blot analysis can be done at final concentrations of 0.25-0.5 μg/mL; recognizes a single band in membrane preparations of rat hippocampal neurons ~170,000-180,000 under reduced, SDS PAGE. AB1548 can be used for immunocytochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations recommended are 0.5-1.0 μg/mL.

The antibody can be used for immunopreciptation of detergent solubilized receptor from brain or transfected cells. For most cases 5 μg of antibody is used with immobilized Protein A.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Western blot analysis of transfected cells shows the antibody recognizes both NR2A and NR2B subunits equally. There may be a very slight recognition of NR2C and NR2D. Western blot analysis of rat brain shows a single band comigrating with NMDAR2A and NMDAR2B expressed in transfected cells. AB1548 has also been used effectively in Western blot of the membrane fraction of rabbit cortex and avian brain homogenates. No cross-reaction is seen with NMDAR1 or other glutamate receptor subunits.

Physical form

Affinity purified using the immunogen peptide. Lyophilized from PBS containing 1% BSA.



Note: lot 0509010940 was shipped in liquid format; Do NOT add additional water to this lot of material.

Preparation Note

Maintain lyophilized material at 2-8°C or -20°C. Reconstitute to 500 μL with sterile distilled water. Store reconstituted material frozen (-20°C or -80°C) in undiluted aliquots for up to six months. Avoid repeated thawing and refreezing. Diluted antibody containing 0.1% sodium azide for Western blot analysis can be stored for several weeks and reused repeatedly.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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10 - Combustible liquids

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High prevalence of NMDA receptor IgA/IgM antibodies in different dementia types.
Doss, S; Wandinger, KP; Hyman, BT; Panzer, JA; Synofzik, M; Dickerson, B; Mollenhauer et al.
Annals of Clinical and Translational Neurology null
Grigory Krapivinsky et al.
Neuron, 40(4), 775-784 (2003-11-19)
The NMDA subtype of glutamate receptors (NMDAR) at excitatory neuronal synapses plays a key role in synaptic plasticity. The extracellular signal-regulated kinase (ERK1,2 or ERK) pathway is an essential component of NMDAR signal transduction controlling the neuroplasticity underlying memory processes
Vanessa Lage-Rupprecht et al.
eLife, 9 (2020-12-01)
In the rodent olfactory bulb the smooth dendrites of the principal glutamatergic mitral cells (MCs) form reciprocal dendrodendritic synapses with large spines on GABAergic granule cells (GC), where unitary release of glutamate can trigger postsynaptic local activation of voltage-gated Na+-channels
GluN2B in corticostriatal circuits governs choice learning and choice shifting.
Brigman, JL; Daut, RA; Wright, T; Gunduz-Cinar, O; Graybeal, C; Davis, MI; Jiang et al.
Nature Neuroscience null
Mackenzie Smith et al.
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics, 19(4), 1340-1352 (2022-06-08)
Rett syndrome (RTT) is a neurodevelopmental disorder that is characterized by developmental regression, loss of communicative ability, stereotyped hand wringing, cognitive impairment, and central apneas, among many other symptoms. RTT is caused by loss-of-function mutations in a methyl-reader known as
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Glutamate is an excitatory neurotransmitter found in the synaptic vesicles of glutamatergic synapses. The post-synaptic neurons in these synapses contain ionotropic and metabotropic glutamate receptors. Glutamate binds to AMPA (α-amino-3-hydroxy-5- methylisoxazole-4-propionic acid) subtype glutamate receptors, leading to sodium influx into the post-synaptic cell and resulting in neuronal excitability and synaptic transmission. The NMDA (N-methyl-d-aspartate) subtype glutamate receptors, on the other hand, regulate synaptic plasticity, and can influence learning and memory. The metabotropic g-protein coupled mGluRs modulate downstream calcium signaling pathways and indirectly influence the synapse’s excitability. The synaptic architecture includes intracellular scaffolding proteins (PSD-95, GRIP), intercellular cell adhesion molecules (NCAMs, N-Cadherins), and a variety of signaling proteins (CaMKII/PKA, PP1/PP2B). Processes critical for synaptic transmission and plasticity are influenced by these molecules and their interactions. When the function of these molecules is disrupted, it leads to synaptic dysfunction and degeneration, and can contribute to dementia as seen in Alzheimer’s disease.

Número de artículo de comercio global

SKUGTIN
AB154804053252287152

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