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Merck

CC12

Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6)

liquid, clone DCS-6, Calbiochem®

Sinónimos:

Anti-Bcl-1, Anti-PRAD-1

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NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
DCS-6, monoclonal
Species reactivity:
human, mouse, rat
Application:
Citations:
23

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Nombre del producto

Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6), liquid, clone DCS-6, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

DCS-6, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, mouse, rat

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

dilution

(Flow Cytometry (5 µg/mL)
Frozen Sections
Immunoblotting (1 µg/mL)
Immunofluorescence (5 µg/mL)
Immunoprecipitation
Neutralization Studies
Paraffin Sections (5 µg/mL, heat pre-treatment required))

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... CCND1(595)
mouse ... Ccnd1(12443)
rat ... Ccnd1(58919)

Analysis Note

Negative Control
trp E (Ab-1)
Positive Control
MCF7, or A431 cells or breast carcinoma tissue

Application

Flow Cytometry (5 µg/ml; Cat. No. CC12, CC12F)

Frozen Sections (see application references)

Immunoblotting (1 µg/ml)

Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F)

Immunoprecipitation (see application references)

Neutralization Studies (see application references)

Paraffin Sections (5 µg/ml, heat pre-treatment required)

Disclaimer

Toxicity: Standard Handling (A)

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS/1 mouse myeloma cells. Recognizes the cyclin D1 protein.
Recognizes cyclin D1 in MCF-7 and A431 cells. Does not react with cyclins D2 and D3.
This Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of Cyclin D1 (Ab-3).

Immunogen

Human
recombinant human cyclin D1

Other Notes

Does not react with cyclins D2 or D3. Staining of paraffin sections can also be detected with pepsin or trypsin pretreatment; light staining will be detected without pretreatment. Do not microwave sections, as this may destroy staining. For immunofluorescence and flow cytometry, we recommend using the FITC conjugate (Cat. No. CC12F). Antibody should be titrated for optimal results in individual systems.
Inaba, T., et al. 1992. Genomics13, 565.
Jiang, W., et al. 1992. Cancer Res.2, 2980.
Pines, J. 1992. Cell Growth Differ.2, 305.
Xiong, Y., et al. 1992. Genomics13, 575.
Xiong, Y., et al. 1992. Cell71, 504.
Motokura, T., et al. 1991. Nature350, 512.
Solomon, M.J., et al. 1990. Cell63, 1013.
Draetta, G., and Beach, D. 1988. Cell54, 17.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Clase de almacenamiento

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Cancer research, 66(11), 5723-5728 (2006-06-03)
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International journal of cancer, 111(1), 17-22 (2004-06-09)
Cyclin E and Cdk2 have been shown to play an important role in G1/S transition of the cell cycle. Two E-type cyclins (E1 and E2) have been identified to date and share functionally similarities. Upregulation of these cyclins has been
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Kathryn Balmanno et al.
Molecular and cellular biology, 23(24), 9303-9317 (2003-12-04)
Elevation of cellular cyclic AMP (cAMP) levels inhibits cell cycle reentry in a variety of cell types. While cAMP can prevent the activation of Raf-1 and extracellular signal-regulated kinases 1 and 2 (ERK1/2) by growth factors, we now show that
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International journal of oncology, 57(3), 721-732 (2020-07-25)
Pancreatic cancer is associated with a poor prognosis due to challenges in early detection, severe progression of the primary tumor, metastatic lesions, and resistance to antitumor agents. However, previous studies have indicated a relationship between the microbiome and pancreatic cancer
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