CP01
Anti-Actin (Ab-1) Mouse mAb (JLA20)
liquid, clone JLA20, Calbiochem®
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.43
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
ascites fluid
tipo de anticuerpo
primary antibodies
clon
JLA20, monoclonal
Formulario
liquid
contiene
≤0.1% sodium azide as preservative (actin antibody only, CP01-100UL)
reactividad de especies (predicha por homología)
all
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
OK to freeze
dilution
(Immunoblotting (1:5000-1:20000)
Immunofluorescence (1:10000,
Recomended dilution for secondary antibody (0.05 µg/mL, 1:2000 dilution))
isotipo
IgM
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... ACTB(60)
Descripción general
Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.
Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.
Recognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1 Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN Entrez Gene Name: actin, alpha 1, skeletal muscle Hu Entrez ID: 58, 71 (Related Antibodies: CP64) Mu Entrez ID: 11459 ;-; 11465 Rat Entrez ID: 29437 ;-; 287876
Inmunógeno
Chicken
chicken cytoskeletal proteins
Aplicación
Immunoblotting (1:5000-1:20000)
Immunofluorescence (1:10000, see application references)
Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)
Immunofluorescence (1:10000, see application references)
Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)
Forma física
100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.
Nota de preparación
Following initial thaw, aliquot ascites and freeze (-20°C).
Nota de análisis
Positive Control
All animal cell lines
All animal cell lines
Otras notas
Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Lin, J.J. 1981. Proc. Natl. Acad. Sci.78, 2335.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.
Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Lin, J.J. 1981. Proc. Natl. Acad. Sci.78, 2335.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
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Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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