Documentos clave
MAB2160
Anti-Fragile X Mental Retardation Protein Antibody, clone 1C3
ascites fluid, clone 1C3, Chemicon®
Sinónimos:
FMRP
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
origen biológico
mouse
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
ascites fluid
tipo de anticuerpo
primary antibodies
clon
1C3, monoclonal
reactividad de especies
mouse, human, rat
fabricante / nombre comercial
Chemicon®
técnicas
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotipo
IgG1κ
idoneidad
not suitable for flow cytometry
not suitable for immunoprecipitation
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... FRAXA(2477), FRAXA(108684022)
Descripción general
Fragile X Mental Retardation Protein (FMRP) is a RNA-binding protein that is associated to polysomes and may be involved in the transport of mRNA from the nucleus to the cytoplasm. Defects in FMR1 are the cause of Fragile X syndrome, which is a common genetic disease characterized by moderate to severe mental retardation, macroorchidism, large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.
~71 kDa
Inmunógeno
Fusion protein with a full length FMRP (human).
Aplicación
Anti-Fragile X Mental Retardation Protein Antibody, clone 1C3 detects level of Fragile X Mental Retardation Protein & has been published & validated for use in ELISA, IC, IH, IH(P) & WB.
Immunohistochemistry:
Frozen and paraffin sections. 1:500-1:5,000 dilution of a previous lot was used.
ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.
Detection of FMRP on Blood Smears:
A 1:500-1:5,000 dilution of a previous lot was used.
Immunocytochemistry:
A 1:500-1:5,000 dilution of a previous lot was used on transfected cells. Light fixation (2% PFA, permeabilize with 0.1% triton in block only)
Optimal working dilutions must be determined by the end user.
Frozen and paraffin sections. 1:500-1:5,000 dilution of a previous lot was used.
ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.
Detection of FMRP on Blood Smears:
A 1:500-1:5,000 dilution of a previous lot was used.
Immunocytochemistry:
A 1:500-1:5,000 dilution of a previous lot was used on transfected cells. Light fixation (2% PFA, permeabilize with 0.1% triton in block only)
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
RNA Binding Protein (RBP)
Neurodegenerative Diseases
RNA Binding Protein (RBP)
Acciones bioquímicas o fisiológicas
Human and mouse FMRP. The epitope is localized in the N-terminal half of FMRP. Cross reaction with FXR protein may be detected in cases of high expression of the latter proteins.
Forma física
Ascites mouse monoclonal IgG1k fluid containing no preservatives
Unpurified
Nota de preparación
Stable for 1 year at -20ºC from date of receipt.
Nota de análisis
Control
HeLa whole cell lysate, HeLa nuclear lysate, mouse E17 spinal cord lysate
HeLa whole cell lysate, HeLa nuclear lysate, mouse E17 spinal cord lysate
Evaluated by Western Blot on Mouse E17 spinal cord lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected Fragile X Mental Retardation Protein on 10 μg of Mouse E17 spinal cord lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected Fragile X Mental Retardation Protein on 10 μg of Mouse E17 spinal cord lysates.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity.
Bartley, CM; O'Keefe, RA; Bordey, A
Testing null
Gabi Schutzius et al.
Journal of neurodevelopmental disorders, 5(1), 8-8 (2013-04-04)
Hypermethylation of the fragile X mental retardation 1 gene FMR1 results in decreased expression of FMR1 protein FMRP, which is the underlying cause of Fragile X syndrome - an incurable neurological disorder characterized by mental retardation, anxiety, epileptic episodes and
Julie B Zang et al.
PLoS genetics, 5(12), e1000758-e1000758 (2009-12-17)
The mental retardation, autistic features, and behavioral abnormalities characteristic of the Fragile X mental retardation syndrome result from the loss of function of the RNA-binding protein FMRP. The disease is usually caused by a triplet repeat expansion in the 5'UTR
The RNA-binding protein fragile X-related 1 regulates somite formation in Xenopus laevis.
Huot, ME; Bisson, N; Davidovic, L; Mazroui, R; Labelle, Y; Moss, T; Khandjian, EW
Molecular Biology of the Cell null
Beata Stepniak et al.
EMBO molecular medicine, 7(12), 1565-1579 (2015-11-28)
Fragile X syndrome (FXS) is mostly caused by a CGG triplet expansion in the fragile X mental retardation 1 gene (FMR1). Up to 60% of affected males fulfill criteria for autism spectrum disorder (ASD), making FXS the most frequent monogenetic
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| MAB2160 | 04053252266911 |
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