Anti-Cytokeratin AE1/AE3 Antibody, recognizes acidic & basic cytokeratins, clone AE1/AE3

40-70 kDa

Keratins are a group of water-insoluble proteins that form monofilaments, a class of intermediate filament. These filaments form part of the cytoskeletal complex in epidermis and in most other epithelial tissues. Nineteen human epithelial keratins are resolved with two-dimensional gels electrophoresis (Moll et al., 1982). These can be divided into acid (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50′, 48, 46, 45, and 40 kDa. The basic keratins have molecular weights of 65-67, 64, 59, 58, 56, and 52 kDa.Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state (Sun et al., 1984; Cooper et al., 1985; Sun et al., 1985);The 56.5/65-67 kDa pair is present in keratinized (differentiated) epidermis;The 55/64 kDa pair is characteristic of normal (corneal-type) epithelial differentiation (Moll et al., 1982; Sun et al., 1984);The 51/59 kDa pair is characteristic of the stratified squamous epithelial of internal organism such as esophagus and tongue (Moll et al., 1982; Cooper et al., 1985);The 51/58 kDa pair is a keratinocyte marker; the pair is present in almost all stratified epithelia irrespective of the state of cellular stratification (Moll et al., 1982; Sun et al., 1984);The 48/56 kD pair is characteristic of hyperproliferative (de-differentiated) keratinocytes (Moll et al., 1982; Weiss et al., 1984)t;The 45/52 kD pair and to a lesser extent, the 46/54 kDa pair are characteristic of simple epithelia (Moll et al., 1982);The 40 kD keratin is present in most epithelia except adult epidermis (Moll et al., 1982).

Epitope: Recognizes acidic & basic cytokeratins

Human epidermal keratins

Anti-Cytokeratin AE1/AE3 Antibody, recognizes acidic & basic cytokeratins, clone AE1/AE3 is an antibody against Cytokeratin AE1/AE3 for use in ELISA, IH & WB.

ELISA:
A previous lot of this antibody was used for ELISA (Woodcock-Mitchel & Sun, 1982).

Western blot:
(Woodcock-Mitchel & Sun, 1982; Tseng et al., 1982; Laster et al., 1986) ELISA: (Woodcock-Mictchel & Sun, 1982)

Immunohistochemistry(paraffin):
0.5-2 µg/mL of a previous lot was used on staining of unfixed frozen or formalin -fixed, paraffin-embedded tissue section (Woodcock-Mitchel & Sun, 1982; Tseng et al., 1982; Asch * Asch, 1986; Rodriguez et al., 1986; Clausen et al., 1986; Klein-Szanto et al., 1987; Reibel et al., 1985). Trypsin or pepsin digestion is required for proper staining on paraffin embedded tissues. {Trypsin 1 mg/mL 10 minutes, 37°C, or pepsin 1 mg/mL 5 minutes 37°C}. High temperature with citrate antigen retrieval can also be used.

Optimal dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Cytokeratins

The stringent, but broad, specificity of pooled AE1/AE3 antibody has made this preparation very useful as a general stain for normal and neoplastic cells of epithelial origin. Anti-Keratin AE1 recognizes the 56.5, 50, 50’, 48, and 40 kD keratins of the acidic subfamily. Anti-keratin AE3 recognizes all members of the basic subfamily {65,67,64,59.59,56,52 human basic cytokeratins}.

Anti-keratin AE1/AE3 is supplied in 0.5 mL borate-buffered saline, pH 8.0, containing 0.09% sodium azide

Format: Purified

Sodium sulfate precipitation

Stable for 1 year at 2-8ºC from date of receipt.
DO NOT FREEZE.

Control
All epithelium-derived tissues & tumors

Routinely evaluated by Western Blot on A431 lysates.

Western blot:
1:500 dilution of this lot detected CYTOKERATIN AE1/AE3 on 10 μg of A431 lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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