Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9

Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.

Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

Reacts with Heterochromatin protein-1 b (HP1b).

Recombinant mouse HP1beta.

Use Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9 (Mouse Monoclonal Antibody) validated in ELISA, ICC, IHC, IP, WB to detect Heterochromatin Protein-1 β also known as HP1b.

Western blot: 1:500-1:5,000


Immunofluorescence: 1:500-1:5,000


Immunohistochemistry: 1:500-1:5,000


Immunoprecipitation: 1:500 - 1:5,000


ELISA: 1:500-1:5,000


Optimal working dilutions must be determined by the end user.

28 kDa

Control
HeLa, 293 or NIH/3T3 cell lysates

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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