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Merck

MAB3864

Anti-DNA/Histone H1 Antibody

Chemicon®, from mouse

Sinónimos:

Anti-H1.4, Anti-H1E, Anti-H1F4, Anti-H1s-4, Anti-HIST1H1E, Anti-RMNS, Anti-dJ221C16.5

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
monoclonal
Application:
ELISA, FACS, ICC, IHC
Citations:
39
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biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... H1-1(3024)

Categorías relacionadas

Immunogen

Human Raji cell nuclei

Application

Anti-DNA/Histone H1 Antibody is a high quality Mouse Monoclonal Antibody for the detection of DNA/Histone H1 & has been validated in ELISA, FC, ICC & IHC.
Immunocytochemistry: 5-10 μg/mL on 2% paraformaldehyde fixed cells (10 minutes at room temperature)

Immunohistochemistry: 5-10 μg/mL on paraformaldehyde or B5 fixed tissue sections.

Flow cytometry: 5-10 μg/mL using 2% paraformaldehyde fixed cells.

ELISA, direct and indirect

Optimal working dilutions must be determined by end user.

STAINING PATTERN:

Nuclear staining observed. Dense staining of nuclei. No staining of nucleoli.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Biochem/physiol Actions

Reacts with DNA/Histone 1. The antibody can be used to stain nuclei of all species and to identify necrosis. By immunohistochemistry, the antibody shows dense staining of nuclei with no staining of nucleoli observed.

Physical form

Format: Purified
Protein A purified culture supernatant. Liquid in PBS

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Gang Liu et al.
Mucosal immunology, 12(4), 862-873 (2019-04-13)
Extra-intestinal manifestations (EIM) are common in inflammatory bowel disease (IBD). One such EIM is sub-clinical pulmonary inflammation, which occurs in up to 50% of IBD patients. In animal models of colitis, pulmonary inflammation is driven by neutrophilic infiltrations, primarily in
Neutrophil Responses to Sterile Implant Materials.
Jhunjhunwala, S; Aresta-DaSilva, S; Tang, K; Alvarez, D; Webber, MJ; Tang, BC; Lavin et al.
Testing null
Simona Grasso et al.
Thrombosis research, 161, 36-42 (2017-11-28)
The circulating zymogen form of Factor VII activating protease (FSAP) can be activated by histones and nucleosomes in vivo. These cell-death-associated nuclear factors are also actively extruded into the extracellular space by neutrophils through a process called neutrophil extracellular trap
Verena Peek et al.
Pharmaceuticals (Basel, Switzerland), 14(6) (2021-07-03)
High mobility group box (HMGB)1 action contributes to late phases of sepsis, but the effects of increased endogenous plasma HMGB1 levels on brain cells during inflammation are unclear. Here, we aimed to further investigate the role of HMGB1 in the
Nicole de Buhr et al.
Frontiers in microbiology, 9, 966-966 (2018-06-06)
Granulocytes mediate the first line of defense against infectious diseases in humans as well as animals and they are well known as multitasking cells. They can mediate antimicrobial activity by different strategies depending on the pathogen they encounter. Besides phagocytosis

Número de artículo de comercio global

SKUGTIN
MAB386404053252612503

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