Anti-Tom20/Tomm20 Antibody, clone 2F8.1

Mitochondrial import receptor subunit TOM20 homolog (TOM20 or TOMM20), also known as mitochondrial 20 kDa outer membrane protein or outer mitochondrial membrane receptor Tom20, is a central component of the pre-protein translocase complex of the outer mitochondrial membrane (TOM complex) which is responsible for the recognition and translocation of cytosolically synthesized mitochondrial pre-proteins. The TOM complex consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). TOM20 interacts with TOM22 and facilitates the movement of pre-proteins into the TOM40 translocation pore. Also interacts with APEX1.

GST-tagged recombinant protein corresponding to human Tom20/Tomm20.

Anti-Tom20/Tomm20, clone 2F8.1 detects levels of Tom20/Tomm20 proteins & has been published & validated for use in WB & ICC.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Tom20/Tomm20 in A431, NIH/3T3, and HeLa cells.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Tom20/Tomm20 in 200 µg of HeLa cell lysate.

~ 16 kDa observed

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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