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Merck

OP07

Anti-v-Src (Ab-1) Mouse mAb (327)

liquid, clone 327, Calbiochem®

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NACRES:
NA.41
UNSPSC Code:
12352203

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Nombre del producto

Anti-v-Src (Ab-1) Mouse mAb (327), liquid, clone 327, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

327, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, human, rat

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

dilution

(Immunoblotting (2.5 µg/mL)
Immunoprecipitation (1 µg antibody)
Frozen Sections (not recommended)
Immunofluorescence (not recommended)
Paraffin Sections (not recommended))

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... SRC(6714)

Analysis Note

Positive Control
NIH/3T3 cells

Application

Immunoblotting (2.5 µg/ml, see application references)

Immunoprecipitation (1 µg antibody or use Cat. No. OP07A; see application references)

Frozen Sections (not recommended)

Immunofluorescence (not recommended)

Paraffin Sections (not recommended)

Disclaimer

Toxicity: Standard Handling (A)

General description

Purified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 myeloma cells (see application references). Recognizes the ~60 kDa Src protein.
Recognizes the ~60 kDa v-Src and c-Src proteins in NIH/3T3 and HT1080 cells. May appear as a doublet due to phosphorylation.
This Anti-v-Src (Ab-1) Mouse mAb (327) is validated for use in Immunoblotting, Immunoprecipitation, Frozen Sections, Immunofluorescence, Paraffin Sections for the detection of v-Src (Ab-1).

Immunogen

Epitope: within the SH3 domain of v-Src and c-Src
purified v-Src

Other Notes

Courtneidge, S.A. and Heber, A. 1987. Cell50, 1031.
Cartwright, C.A., et al. 1985. Mol. Cell. Biol.5,, 2647.
Courtneidge, S.A. 1985. EMBO J.4, 1471.
Whitman, M., et al. 1985. Nature315, 239
Bolen, J.B., et al. 1984. Cell38, 767.
Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci.77, 1311.
Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci.76, 3159.
Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci.75, 2021.
Collett, M.S., et al. 1978. Cell15, 1363.
pp60src may appear as a doublet due to phosphorylation. This antibody can be used to precipitate active src kinase (see application references). The agarose conjugate, Cat. No. OP07A, is available separately for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Clase de almacenamiento

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Anna S Urbanska et al.
Scientific reports, 7(1), 1876-1876 (2017-05-14)
Local protein synthesis occurs in axons and dendrites of neurons, enabling fast and spatially restricted responses to a dynamically changing extracellular environment. Prior to local translation, mRNA that is to be translated is packed into ribonucleoprotein particles (RNPs) where RNA
Arkadiusz Welman et al.
Neoplasia (New York, N.Y.), 8(11), 905-916 (2006-11-30)
The levels and activity of c-Src in colorectal cancer cells increase steadily during the course of colorectal carcinogenesis and are most highly elevated in advanced metastatic disease. However, the effects of increases in c-Src activity on the proliferation of colorectal
Soshi Noshita et al.
Journal of cell science, 136(15) (2023-07-13)
The non-receptor tyrosine kinase SRC is overexpressed and/or hyperactivated in various human cancers, and facilitates cancer progression by promoting invasion and metastasis. However, the mechanisms underlying SRC upregulation are poorly understood. In this study, we demonstrate that transforming growth factor-β
Lisa M Mehlmann et al.
Reproduction (Cambridge, England), 129(5), 557-564 (2005-04-28)
SRC family kinases (SFKs) function in initiating Ca2+ release at fertilization in several species in the vertebrate evolutionary line, but whether they play a similar role in mammalian fertilization has been uncertain. We investigated this question by first determining which
L Dulabon et al.
Neuron, 27(1), 33-44 (2000-08-12)
Mice that are mutant for Reelin or Dab1, or doubly mutant for the VLDL receptor (VLDLR) and ApoE receptor 2 (ApoER2), show disorders of cerebral cortical lamination. How Reelin and its receptors regulate laminar organization of cerebral cortex is unknown.
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