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Merck

10102121001

Roche

Adenosine Deaminase (ADA)

from calf intestine

Sinónimos:

ADA, Adenosine Deaminase

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UNSPSC Code:
12352204
Número CE:
NACRES:
NA.54
Specific activity:
~200 units/mg protein (At 25 °C with adenosine as the substrate.)
Biological source:
bovine intestine
Servicio técnico
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biological source

bovine intestine

form

suspension

specific activity

~200 units/mg protein (At 25 °C with adenosine as the substrate.)

packaging

pkg of 1 mL (10 mg)

manufacturer/tradename

Roche

technique(s)

activity assay: suitable

color

white

optimum pH

7.0-7.4

solubility

water: miscible

suitability

suitable for chemiluminescence

NCBI accession no.

UniProt accession no.

application(s)

life science and biopharma

foreign activity

AMP-deaminase <0.01%, AP <0.01% (with pre incubation), Guanase <0.01%, NP <0.01%

storage temp.

2-8°C

Gene Information

bovine ... ADA(280712)

General description

Adenosine aminohydrolase.
Adenosine deaminase (ADA) is a zinc metalloenzyme.

Application

Deamination of adenosine analogs to the corresponding inosine analogs
Adenosine deaminase (ADA) has been used in lipolysis assay along with other reagents.
Chemiluminescent adenosine assay.

Biochem/physiol Actions

Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Small particles are normal. Before using stir up the precipitate and proceed.

Analysis Note

Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each

Other Notes

For life science research only. Not for use in diagnostic procedures.


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Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

No data available

flash_point_c

No data available



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Cultured astrocytes do not release adenosine during hypoxic conditions.
Fujita T, et al.
Journal of Cerebral Blood Flow and Metabolism, 32(1), e1-e7 (2012)
Adenosine deaminase from calf intestinal mucosa.
C A ZITTLE
The Journal of biological chemistry, 166(2), 499-503 (1946-12-01)
Mi-Jeong Lee et al.
Methods in enzymology, 538, 49-65 (2014-02-18)
Obesity is reaching epidemic proportions so there is growing interest in the mechanisms that regulates adipose tissue development and function. Although murine adipose cell lines are useful for many mechanistic studies, primary human adipose stromal cells (ASCs), which can be