RDD035
HEPES sodium salt
≥99.5% (titration), free-flowing, Redi-Dri™
Sinónimos:
HEPES Sodium, HEPES hemisodium salt, Sodium HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
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Acerca de este artículo
Fórmula empírica (notación de Hill):
C8H17N2NaO4S
Número CAS:
Peso molecular:
260.29
UNSPSC Code:
12161700
NACRES:
NA.25
MDL number:
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Permítanos ayudarleInChI
1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
SMILES string
[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
InChI key
RDZTWEVXRGYCFV-UHFFFAOYSA-M
product line
Redi-Dri™
assay
≥99.5% (titration)
quality
free-flowing
useful pH range
6.8-8.2
pKa (25 °C)
7.5
application(s)
diagnostic assay manufacturing
Quality Level
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General description
HEPES sodium salt is essential to prepare HEPES buffer that is commonly used for cell culture and protein applications. It is useful for maintaining homeostasis when working with cells outside of the incubator by balancing CO2. HEPES buffering capacity exceeds most buffers, with a pKa of 7.5, making it optimal for maintaining pH in biological systems. In solution, it is non-toxic, zwitterion with a physiological buffering range of pH 6.8 - 8.2. It has a low metal binding capacity and is safe to use in research for metal-binding proteins.
Application
HEPES sodium salt can be used:
- as a protein precipitation buffer
- in cell culture media
- as a binding buffer
Features and Benefits
- Redi-Dri™ eliminates clumping for hygroscopic chemicals
- powder remains free-flowing and easy to use
- Safer to handle and weigh out for standard use in lab
- Eliminates clumps, maintains product moisture, quality, and characteristics
Physical form
Lyophilized, spray dried, or crystallized material.
Other Notes
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Legal Information
Redi-Dri is a trademark of Sigma-Aldrich Co. LLC
Clase de almacenamiento
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Peng Yang
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When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue"
Jihye Park et al.
The Analyst, 137(19), 4411-4414 (2012-08-07)
We have developed a quickly responsive, and specific fluorescent assay for the detection of Au(III) on the basis of the formation of gold nanoparticles in the presence of HEPES, which cleave the C-I bond of I-BODIPY 1 to yield the
S Hugel et al.
British journal of pharmacology, 166(8), 2402-2416 (2012-03-29)
Artificial buffers such as HEPES are extensively used to control extracellular pH (pH(e) ) to investigate the effect of H(+) ions on GABA(A) receptor function. In neurones cultured from spinal cord dorsal horn (DH), dorsal root ganglia (DRG) and cerebellar
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