75632
Osmium tetroxide solution
suitable for electron microscopy, 4% in H2O
Sinónimos:
Osmium(IV) oxide
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Fórmula lineal:
OsO4
Número CAS:
Peso molecular:
254.23
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
Form:
solution
Servicio técnico
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solution
Quality Level
reaction suitability
reagent type: oxidant
concentration
4% in H2O
technique(s)
electron microscopy: suitable
color
colorless to yellow
pH
6.0-8.0
density
1.0 g/mL at 20 °C
application(s)
hematology
histology
storage temp.
2-8°C
SMILES string
O=[Os](=O)(=O)=O
InChI
1S/4O.Os
InChI key
VUVGYHUDAICLFK-UHFFFAOYSA-N
Application
Osmium tetroxide solution has been used as a fixative in scanning and transmission electron microscopy.
Biochem/physiol Actions
Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.
Packaging
5 mL in ampule
10 mL in ampule
10 mL in ampule
Other Notes
Due to the hazardous nature of osmium tetroxide, it is recommended to use an ampule opener when using this chemical.
signalword
Danger
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
Clase de almacenamiento
6.1B - Non-combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Bozzola JJ and Russell LD
Electron Microscopy: Principles and Techniques for Biologists (1999)
Antibacterial effect of component of Terminalia muelleri Benth. Against Staphylococcus aureus.
International Journal of Pharmaceutics, 6, 407-412 (2010)
G C Glikin et al.
Nucleic acids research, 12(3), 1725-1735 (1984-02-10)
Supercoiled plasmids Col E1 and cDm 506 (a Col E1 derivative carrying the D. melanogaster histone gene repeat) were treated with OsO4 in presence of pyridine and the reaction products were analyzed using different approaches. Gel electrophoresis showed that OsO4