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Merck

83200

Pyronin Y

suitable for microscopy

Sinónimos:

Pyronin G, Pyronin J

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Acerca de este artículo

Fórmula empírica (notación de Hill):
C17H19ClN2O
Número CAS:
Peso molecular:
302.80
EC Number:
202-147-8
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Colour Index Number:
45005
Beilstein/REAXYS Number:
3795789
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Nombre del producto

Pyronin Y, suitable for microscopy

InChI key

INCIMLINXXICKS-UHFFFAOYSA-M

InChI

1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1

SMILES string

[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C

mp

250-260 °C (lit.)

solubility

water: 0.01 g/10 mL, red

εmax

≥1650 at 543-553 nm in 50% ethanol
≥650 at 249-259 nm in 50% ethanol

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

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Biochem/physiol Actions

Pyronin Y is important in histochemistry and is combined with methyl green for staining of RNA. The mixture results in bright red/green staining of nucleic acids.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Has Pyronin Y, Product 83200, been use-tested for the demonstration of RNA?

    No. There are, however, two other Pyronin products from Sigma-Aldrich which should be suitable.  Product No. P9172 has been used to stain RNA in polyacrylamide gels.  Product No. 213519 was submitted to the Biological Stain Commission for certification.  Testing was performed by the Biological Stain Commission to ensure the Pyronin Y stained RNA in tissue sections.

  6. Is there a prepared solution of Methyl Green and Pyronin Y for the demonstration of plasma cells and RNA in tissue sections?

    Yes, product HT70116 is available in pre-made form.  The product is approved for in vitro diagnostic (IVD) use.

  7. What is the emission wavelength of Pyronin Y, Product 83200?

    The emission is at 570 nm when dissolved in water.

  8. What is the solubility of Pyronin Y, Product 83200, in water and ethanol?

    Solubility is not tested at Sigma Aldrich, but the literature reports solubility up to 9% (i.e., 90 mg/mL) in water and 0.6% (6 mg/mL) in ethanol.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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