A0512
Amylose from potato
used as amylase substrate
Sinónimos:
α-Amylose
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SMILES string
O1[C@@H]([C@@H]([C@H]([C@@H]([C@H]1CO)O[C@H]3O[C@@H]([C@H]([C@@H]([C@H]3O)O)O)CO)O)O)O[C@@H]2[C@H](O[C@@H]([C@@H]([C@H]2O)O)O)CO
InChI
1S/C18H32O16/c19-1-4-7(22)8(23)12(27)17(31-4)34-15-6(3-21)32-18(13(28)10(15)25)33-14-5(2-20)30-16(29)11(26)9(14)24/h4-29H,1-3H2/t4-,5-,6-,7-,8+,9-,10-,11-,12-,13-,14-,15-,16+,17-,18-/m1/s1
InChI key
FYGDTMLNYKFZSV-PXXRMHSHSA-N
biological source
potato
form
powder
impurities
amylopectin, essentially free, ≤10% ethanol, ≤2% butanol
color
white to off-white
solubility
0.05 M NaOH: 1 mg/mL, clear to slightly hazy, colorless
storage temp.
room temp
Quality Level
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General description
Application
Biochem/physiol Actions
Other Notes
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What can I use to solubilize Amylose from Potato, Product A0512?
This product is soluble in 50 mM NaOH at a concentration of 1 mg/mL. The product can also be dissolved as follows (final concentration = 1 mg/ml): Dissolve 100 mg in 1 mL ethanol. Add 10 mL water and 2 mL of 10% NaOH. Heat in boiling water bath until sample dissolves, cool to ambient temperature, then dilute to 100 mL with water.
What is the blue value of Amylose from Potato, Product A0512? How is it determined?
Prepare iodine solution and Solution A: 1. Iodine solution: 0.2% iodine in 2% potassium iodide.2. Solution A: To 100 mg of sample in 100 mL flask, add 1 mL ethanol. Add 10 mL water and 2 mL of 10% NaOH. Warm in boiling water bath until sample dissolves. After cooling, dilute to 100 mL with water.Transfer 2.5 mL of solution A to 250 mL flask. To this flask, add 50 mL water, then 2 drops of 6 N hydrochloric acid, followed by 2.5 mL of Iodine solution. Dilute to 250 mL with water. Read absorbance at 680 nm against a blank.The final solution to be read will be at a sample concentration of 10 μg per mL, and 1% of the iodine solution.This procedure is based on McCready, R. M., and Hassid, W. Z., The Separation and Quantitative Estimation of Amylose and Amyolpectin in Potato Starch. J. Am. Chem. Soc., Vol. 65, pp. 1154-1157 (1943).
What is the molecular weight of Amylose from Potato, Product A0512?
Sigma-Aldrich has not determined the molecular weight of the product. One reported range is from 40,000 to 340,000 Daltons, with chains containing 250 to 2000 anhydroglucose units. Source: WHO Food Additives Series No. 5.
What is the method of preparation of Amylose from Potato, Product A0512?
The method of preparation is given in Street and Close, Clin. Chim. Acta, 1, 256-268 (1956). Potato starch is dissolved in boiling 2% (w/v) NaCl solution, filtered while hot, then cooled. Thymol is added, and the mixture is stirred for 48 hr. Amylopectin is decanted away in the supernatant. The precipitate is washed six times with concentrated thymol solution, four times with absolute ethanol, and dried.
What do the "Types" of Amylose from Potato, Product A0512, mean?
The "Type" of the product has no significant meaning outside Sigma-Aldrich. The differences between products were designated by types before Sigma had established product numbers for specific products. The types were differentiated on several bases, including manufacturing method, source, and purity.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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