B1522
Basal Medium Eagle
With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Sinónimos:
BME
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UNSPSC Code:
12352207
NACRES:
NA.75
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Permítanos ayudarleNombre del producto
Basal Medium Eagle, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
L-glutamine: no
NaHCO3: yes
Earle’s salts (5% CO2): yes
shipped in
ambient
storage temp.
2-8°C
Quality Level
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General description
Eagle′s Basal Medium (BME) is one of the most used synthetic cell culture media. The tissue culture association recommends that the term "Eagle′s Basal Medium" be used only to refer to the formula developed to support HeLa cells. BME has previously been used in monolayer culture studies to assess the growth response of normal (WI-38) and transformed (mouse and HeLa) cells. BME is the precursor of Dulbecco′s Modified Eagle′s medium (DME) and Eagle′s Minimum Essential Medium (MEM).
Application
Basal Medium Eagle has been used:
- as a component in the culture medium for cochlear organotypic cultures and salicylate treatment
- to maintain cerebellar granule neurons (CGNs) to determine if there is a system that regulates the distribution and/or group dynamics of axonal mitochondria
- in the preparation of cortical slices, serum-free medium, and slice culture medium for slice overlay assay to study the influence of extracellular signals on neuronal development
Preparation Note
Supplement with 0.292 g/L L-glutamine.
Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Franck Polleux et al.
Science's STKE : signal transduction knowledge environment, 2002(136), pl9-pl9 (2002-06-13)
We have developed a technique that allows characterization and identification of extracellular signals that regulate various aspects of neuronal differentiation. In this in vitro assay, dissociated cells isolated from the developing cerebral wall are labeled and cultured over organotypic cortical
Intermitochondrial signaling regulates the uniform distribution of stationary mitochondria in axons.
Nozomu Matsumoto et al.
Molecular and cellular neurosciences, 119, 103704-103704 (2022-02-09)
In the central nervous system (CNS), many neurons develop axonal arbors that are crucial for information processing. Previous studies have demonstrated that premature axons contain motile and stationary mitochondria, and their balance is important for axonal arborization. However, the mechanisms
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