B7639
(+)-Biotin hydrazide
≥97% (TLC), powder
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Fórmula empírica (notación de Hill):
C10H18N4O2S
Número CAS:
Peso molecular:
258.34
UNSPSC Code:
12352203
NACRES:
NA.46
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
28347
Assay:
≥97% (TLC)
Form:
powder
Servicio técnico
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Permítanos ayudarleQuality Level
assay
≥97% (TLC)
form
powder
solubility
DMSO: ≤20 mg/mL
storage temp.
2-8°C
SMILES string
[H][C@]12CS[C@@H](CCCCC(=O)NN)[C@@]1([H])NC(=O)N2
InChI
1S/C10H18N4O2S/c11-14-8(15)4-2-1-3-7-9-6(5-17-7)12-10(16)13-9/h6-7,9H,1-5,11H2,(H,14,15)(H2,12,13,16)/t6-,7-,9-/m0/s1
InChI key
KOZWHQPRAOJMBN-ZKWXMUAHSA-N
General description
Biotin hydrazide is a biotinylation reagent used to biotinylate glycoproteins with their sugar moieties. Biotin hydrazide can be used to prepare biotin-conjugated alginate. It can also be used for covalent attachment to PAAc via carbodi-imide cross linking.
Application
(+)-Biotin hydrazide has been used:
- the modification of alginate
- for the labelling of mitochondrial proteins from non-muscle tissues
- as a component of glycoprotein staining solution
- in periodic acid-biotin-hydrazide (PABH) assay for mucins
- for labeling surface functional groups, biologically active molecules such as antibodies, lectins, sugars, nucleic acids or molecules with free carboxylic or keto groups.
- for coupling to glycoproteins through the carbohydrate by hydrazone formation
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Boris Polyak et al.
Biomacromolecules, 5(2), 389-396 (2004-03-09)
Biotin was covalently coupled with alginate in an aqueous-phase reaction by means of carbodiimide-mediated activation chemistry to provide a biotin-alginate conjugate for subsequent use in biosensor applications. The synthetic procedure was optimized with respect to pH of the reaction medium
Studying mucin secretion from human bronchial epithelial cell primary cultures
Mucins, 259-277 (2012)
Emma Luong-Van et al.
Biointerphases, 4(2), 13-18 (2010-04-23)
The spatial control of cells on a surface and the patterning of multiple cell types is an important tool for fundamental biological research and tissue engineering applications. A novel technique is described for the controlled seeding of multiple cell types