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Merck

C2888

Acetilcolinesterasa from Electrophorus electricus (electric eel)

Type V-S, lyophilized powder, ≥1,000 units/mg protein

Sinónimos:

AChE, Acetil colinesterasa, Acetilcolina acetilhidrolasa

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About This Item

Número CAS:
9000-81-1
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-559-3
MDL number:
MFCD00081268
Número CE:
3.1.1.7 (BRENDA, IUBMB)

Nombre del producto

Acetilcolinesterasa from Electrophorus electricus (electric eel), Type V-S, lyophilized powder, ≥1,000 units/mg protein

biological source

Electrophorus electricus

type

Type V-S

form

lyophilized powder

specific activity

≥1,000 units/mg protein

mol wt

280 kDa

composition

Protein, ≥45% biuret

pI 

~5.3

solubility

Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)

storage temp.

−20°C

Quality Level

200

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Physical form

Lyophilized powder containing Tris buffer salts

Biochem/physiol Actions

La principal enzima degradadora de la acetilcolina in vivo. Convierte la acetilcolina + H2o en colina + ácido acético.
Acetylcholinesterase (AChE) exhibits its activity against acetylcholine in the neuromuscular junctions and synaptic cleft of cholinergic neurons. It participates in the nitric oxide signaling pathway.

General description

Peso molecular: 280 kDa
Punto isoeléctrico: 5,5
Coeficiente de extinción: E1% = 18,0 (280 nm)

La acetilcolinesterasa de Electrophorus electricus es un tetrámero compuesto de 4 subunidades iguales de 70 kDa cada una. Cada subunidad contiene un sitio activo. La enzima es una glucoproteína que contiene hexosaminas.

Analysis Note

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Application

Acetylcholinesterase from Electrophorus electricus (electric eel) has been used:
  • in acetylcholinesterase activity assay
  • to study its effects on Xenopus embryonic hippocampal cells
  • to study the effect of acetylcholine inhibitors on development and plasticity

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

Disclaimer

For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000515797
0000515796
0000515795
0000515796
0000515797

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Are solutions of Product C2888, Acetylcholinesterase from Electrophorus electricus (electric eel), harmed by freezing?

    Yes, solutions may be harmed by freezing. The data sheet for Product C2888 indicates that solutions stabilized by the addition of BSA are stable when kept refrigerated for up to six months. A customer has reported to us that they tried freezing a solution to which BSA was added; they found that the solution had a 40 percent loss of activity after four months in the freezer. So our best recommendation is that solutions be stabilized with BSA and kept refrigerated, not frozen.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Joana R Almeida et al.
Biomolecules, 10(8) (2020-08-06)
The development of alternative ecological and effective antifouling technologies is still challenging. Synthesis of nature-inspired compounds has been exploited, given the potential to assure commercial supplies of potential ecofriendly antifouling agents. In this direction, the antifouling activity of a series
Silvia Olivera et al.
Molecular and cellular neurosciences, 23(1), 96-106 (2003-06-12)
Here we show that chronic application of low concentrations (0.01-0.05 U/ml) or a single application of 1-5 U/ml acetylcholinesterase (AChE) promotes the extension of neuronal processes, synapse formation, and alpha-amino-3-hydroxy-5-methylisoxazolepropionate receptor (AMPAR) surface expression in both embryonic and postnatal hippocampal
Daniela Pereira et al.
Marine drugs, 19(1) (2020-12-31)
Over the last decades, antifouling coatings containing biocidal compounds as active ingredients were used to prevent biofouling, and eco-friendly alternatives are needed. Previous research from our group showed that polymethoxylated chalcones and glycosylated flavones obtained by synthesis displayed antifouling activity
Daniel A S Kitagawa et al.
Journal of enzyme inhibition and medicinal chemistry, 36(1), 1370-1377 (2021-06-22)
Organophosphorus poisoning caused by some pesticides and nerve agents is a life-threating condition that must be swiftly addressed to avoid casualties. Despite the availability of medical countermeasures, the clinically available compounds lack a broad spectrum, are not effective towards all
Nagham Alatrash et al.
ChemMedChem, 12(13), 1055-1069 (2017-06-13)
Four mononuclear [(L-L)2 Ru(tatpp)]2+ and two dinuclear [(L-L)2 Ru(tatpp)Ru(L-L)2 ]4+ ruthenium(II) polypyridyl complexes (RPCs) containing the 9,11,20,22-tetraazatetrapyrido[3,2-a:2',3'-c:3'',2''-l:2''',3'''-n]pentacene (tatpp) ligand were synthesized, in which L-L is a chelating diamine ligand such as 2,2'-bipyridine (bpy), 1,10-phenanthroline (phen), 3,4,7,8-tetramethyl-1,10-phenanthroline (Me4 phen) or 4,7-diphenyl-1,10-phenanthroline
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