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Merck

C4585

Anti-β-Tubulin−Cy3 antibody, Mouse monoclonal

clone TUB 2.1, purified from hybridoma cell culture

Sinónimos:

Monoclonal Anti-β-Tubulin

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NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
CY3 conjugate
Clone:
TUB 2.1, monoclonal
Application:
IF (d)
Citations:
38

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biological source

mouse

conjugate

CY3 conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

TUB 2.1, monoclonal

form

buffered aqueous solution

mol wt

antigen 55 kDa

species reactivity

human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster

storage condition

protect from light

technique(s)

direct immunofluorescence: 1:100 using cultured chicken fibroblasts or BHK cells

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TUBB(203068), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383)
mouse ... Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb3(22152)
rat ... Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554)

General description

Monoclonal Anti-b-Tubulin (mouse IgG1 isotype) is derived from the TUB2.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with purified rat brain tubulin. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Tubulin is a heterodimer, which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. β-tubulin structure is characterized with the core of two β-sheets enclosed by α-helices.

Immunogen

tubulin from rat brain.

Application

Monoclonal Anti-β-Tubulin-Cy3 antibody produced in mouse has been used in:
  • immunohistochemical assays
  • immunofluorescence
  • western blotting
  • immunocytochemistry
  • immunoblotting
  • immunostaining

Biochem/physiol Actions

Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Mutation in the β-tubulin gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations.
The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Clase de almacenamiento

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000226312
0000226312
0000470978
0000470978
0000160919

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Liang Qiang et al.
Molecular biology of the cell, 21(2), 334-344 (2009-11-27)
The formation of interstitial axonal branches involves the severing of microtubules at sites where new branches form. Here we wished to ascertain whether basic fibroblast growth factor (bFGF) enhances axonal branching through alterations in proteins involved in the severing of
MicroRNA-208a is a regulator of cardiac hypertrophy and conduction in mice
Callis TE, et al.
The Journal of Clinical Investigation, 119(9), 2772-2786 (2009)
M Janmaleki et al.
Scientific reports, 6, 32418-32418 (2016-09-02)
This study focused on the effects of simulated microgravity (s-μg) on mechanical properties, major cytoskeleton biopolymers, and morphology of endothelial cells (ECs). The structural and functional integrity of ECs are vital to regulate vascular homeostasis and prevent atherosclerosis. Furthermore, these
Hong-Ryul Jung et al.
PloS one, 9(3), e91926-e91926 (2014-03-19)
Similar to stem cells, naïve T cells undergo asymmetric division following activation. While asymmetric division of T cells has been shown to be an important mechanism for the generation of lymphocyte fate diversity during immune responses, key factors that influence
Comprehensive genotype-phenotype correlation in lissencephaly
Tan AP, et al.
Quantitative imaging in medicine and surgery, 8(7), 673-673 (2018)
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