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C9426

3-[(3-Colamidopropil)dimetilamonio]-1-propanosulfonato hydrate

Name: 3-[(3-Colamidopropil)dimetilamonio]-1-propanosulfonato hydrate
Brand: SIGMA

BioReagent, suitable for electrophoresis, ≥98% (HPLC)

Sinónimos:

CHAPS

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Fórmula empírica (notación de Hill):

C₃₂H₅₈N₂O₇S · xH₂O

Número CAS:

331717-45-4

Peso molecular:

614.88 (anhydrous basis)

NACRES:

NB.22

PubChem Substance ID:

24892997

UNSPSC Code:

12161902

MDL number:

MFCD00012116

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Propiedades

description

zwitterionic

Quality Level

200

product line

BioReagent

assay

≥98% (HPLC)

form

powder

mol wt

614.88 g/mol (anhydrous basis)

technique(s)

electrophoresis: suitable, protein quantification: suitable

impurities

<0.04% DMF

mp

157 °C (dec.) (lit.)

solubility

water: 50 mg/mL, clear, colorless

suitability

suitable for electrophoresis

storage temp.

room temp

SMILES string

O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C

InChI

1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1

InChI key

SJCUTFKCLFLIFE-JWTJKVBLSA-N

Descripción

General description

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.

Application

CHAPS has been used:

For membrane permeabilization of cells
As a component in lysis buffer for homogenization of liver tissues as well as adipose tissue
As a component in denaturing buffer in enzyme characterization
As a component of 2D- sample buffer in 2D electrophoresis
For protein solubilization in isoelectric focusing