D8168
Anti-Dystrophin Antibody
mouse monoclonal, MANDYS8
Sinónimos:
Dystrophin Antibody, Dystrophin Antibody - Monoclonal Anti-Dystrophin antibody produced in mouse
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Conjugate:
unconjugated
Clone:
MANDYS8, monoclonal
Application:
indirect ELISA
indirect immunofluorescence
microarray
western blot
indirect immunofluorescence
microarray
western blot
Species reactivity:
chicken, rat, human, pig, rabbit, mouse
Citations:
71
Technique(s):
indirect ELISA: suitable
indirect immunofluorescence: 1:400 using frozen human or animal muscle tissue sections.
microarray: suitable
western blot: suitable
indirect immunofluorescence: 1:400 using frozen human or animal muscle tissue sections.
microarray: suitable
western blot: suitable
Uniprot accession no.:
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Permítanos ayudarleNombre del producto
Monoclonal Anti-Dystrophin antibody produced in mouse, clone MANDYS8, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
MANDYS8, monoclonal
species reactivity
chicken, rat, human, pig, rabbit, mouse
technique(s)
indirect ELISA: suitable
indirect immunofluorescence: 1:400 using frozen human or animal muscle tissue sections.
microarray: suitable
western blot: suitable
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... DMD(1756)
mouse ... Dmd(13405)
rat ... Dmd(24907)
Application
Monoclonal Anti-Dystrophin antibody produced in mouse has been used in
- immunohistochemistry
- immunofluorescence
- double immunofluorescence terminal dUTP nick-end labeling (TUNEL)
- immunoblotting
Monoclonal anti-dystrophin antibody can be used for localization of dystrophin using immunochemical assays like ELISA and immunohistochemistry. The antibody can also be used in immunoblotting for brain dystrophin. Further, it can be used in western blotting and double immunofluorescent labelling (diluted 1: 500) of dystrophin.
Biochem/physiol Actions
Dystrophin deficiency is associated with severe Duchenne muscular dystrophy (DMD). Becker muscular dystrophy (BMD) show less pronounced abnormalities of dystrophin protein expression. Since abnormalities in the protein expression occur specifically in patients with these types of muscular dystrophy, dystrophin analysis may be used to distinguish these conditions from other neuromuscular diseases.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Dystrophin (mouse IgG2b isotype) is derived from the MANDYS8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Dystrophin is a structural protein on the inner face of the membrane, consisting of a 25-repeat, rod-like, triple-helical domain separating an N-terminal actin binding domain from two C-terminal domains, one of which is rich in cysteine.
The rod domain of the human dystrophin molecule is present in normal muscle tissue and in nearly all Becker muscular dystrophies. It is absent in the cases of Duchenne muscular dystrophies and in the dystrophic mouse (mdx).
Immunogen
recombinant human dystrophin fragment.
Other Notes
This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200764 Anti-Dystrophin antibody, Mouse monoclonal
clone MANDYS8, purified from hybridoma cell culture
SAB4200764 Anti-Dystrophin antibody, Mouse monoclonal
clone MANDYS8, purified from hybridoma cell culture
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Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Effects of long-term resveratrol-induced SIRT1 activation on insulin and apoptotic signalling in aged skeletal muscle
Sin TK, et al.
Acta Diabetologica, 52(6), 1063-1075 (2015)
The dystrophin complex: structure, function, and implications for therapy
Gao QQ and McNally EM
Comprehensive Physiology, 5(3), 1223-1239 (2011)
Myospryn is a calcineurin-interacting protein that negatively modulates slow-fiber-type transformation and skeletal muscle regeneration
Kielbasa OM, et al.
Faseb Journal, 25(7), 2276-2286 (2011)
Rachel Blitzblau et al.
Brain research, 1218, 21-34 (2008-06-06)
Muscular dystrophy patients often show cognitive impairment, in addition to muscle degeneration caused by dystrophin gene defects. The cognitive impairments lead to speculation that the dystrophin protein family may play a key role at neuronal synapses. Dystrophin regulates the stability
Unacylated ghrelin restores insulin and autophagic signaling in skeletal muscle of diabetic mice
Tam BT, et al.
Pflugers Archiv : European Journal of Physiology, 467(12), 2555-2569 (2015)
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